Special Section on Coherent Raman Imaging Techniques and Biomedical Applications

Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands

[+] Author Affiliations
Chia-Yu Lin, Eric. O. Potma

University of California, Irvine, Department of Chemistry and Beckman Laser Institute, Irvine, California 92697

Jeffrey L. Suhalim

University of California, Irvine, Graduate Program in Mathematical, Computational and Systems Biology, Irvine, California 92697

Chyong Ly Nien, James V. Jester

University of California, Irvine, Gavin Herbert Eye Institute, Irvine, California 92697

Miloš D. Miljković, Max Diem

Northeastern University, Department of Chemistry and Chemical Biology, Boston, Massachusetts 02115

J. Biomed. Opt. 16(2), 021104 (February 09, 2011). doi:10.1117/1.3533716
History: Received June 28, 2010; Revised August 19, 2010; Accepted August 23, 2010; Published February 09, 2011; Online February 09, 2011
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The lipid distribution in the mouse meibomian gland was examined with picosecond spectral anti-Stokes Raman scattering (CARS) imaging. Spectral CARS data sets were generated by imaging specific localized regions of the gland within tissue sections at consecutive Raman shifts in the CH2 stretching vibrational range. Spectral differences between the location specific CARS spectra obtained in the lipid-rich regions of the acinus and the central duct were observed, which were confirmed with a Raman microspectroscopic analysis, and attributed to meibum lipid modifications within the gland. A principal component analysis of the spectral data set reveals changes in the CARS spectrum when transitioning from the acini to the central duct. These results demonstrate the utility of picosecond spectral CARS imaging combined with multivariate analysis for assessing differences in the distribution and composition of lipids in tissues.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Chia-Yu Lin ; Jeffrey L. Suhalim ; Chyong Ly Nien ; Miloš D. Miljković ; Max Diem, et al.
"Picosecond spectral coherent anti-Stokes Raman scattering imaging with principal component analysis of meibomian glands", J. Biomed. Opt. 16(2), 021104 (February 09, 2011). ; http://dx.doi.org/10.1117/1.3533716


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