Special Section on Coherent Raman Imaging Techniques and Biomedical Applications

Optical parametric oscillator-based light source for coherent Raman scattering microscopy: practical overview

[+] Author Affiliations
Sophie Brustlein, Patrick Ferrand, Nico Walther, Sophie Brasselet, Hervé Rigneault

Aix-Marseille Université, CNRS, Institut Fresnel, Ecole Centrale Marseille, Campus de Saint Jérôme, Marseille, 13397 Cedex 20, France

Cyrille Billaudeau, Didier Marguet

Centre d’Immunologie de Marseille-Luminy, INSERM, UMR-S 631, Marseille, 13009 France

CNRS, UMR 6102, Marseille, 13009 France

Université de la Méditerranée, UM 631, Marseille, 13009 France

J. Biomed. Opt. 16(2), 021106 (February 25, 2011). doi:10.1117/1.3533311
History: Received June 16, 2010; Revised September 08, 2010; Accepted September 13, 2010; Published February 25, 2011; Online February 25, 2011
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We present the assets and constraints of using optical parametric oscillators (OPOs) to perform point scanning nonlinear microscopy and spectroscopy with special emphasis on coherent Raman spectroscopy. The difterent possible configurations starting with one OPO and two OPOs are described in detail and with comments that are intended to be practically useful for the user. Explicit examples on test samples such as nonlinear organic crystal, polystyrene beads, and fresh mouse tissues are given. Special emphasis is given to background-free coherent Raman anti-Stokes scattering (CARS) imaging, including CARS hyperspectral imaging in a fully automated mode with commercial OPOs.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Sophie Brustlein ; Patrick Ferrand ; Nico Walther ; Sophie Brasselet ; Cyrille Billaudeau, et al.
"Optical parametric oscillator-based light source for coherent Raman scattering microscopy: practical overview", J. Biomed. Opt. 16(2), 021106 (February 25, 2011). ; http://dx.doi.org/10.1117/1.3533311


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