Special Section on Coherent Raman Imaging Techniques and Biomedical Applications

Comparison of the sensitivity and image contrast in spontaneous Raman and coherent Stokes Raman scattering microscopy of geometry-controlled samples

[+] Author Affiliations
Hyunmin Kim, Garnett W. Bryant

National Institute of Standards and Technology, Gaithersburg, Maryland 20899

University of Maryland, Joint Quantum Institute, College Park, Maryland 20742

Chris A. Michaels, Stephan J. Stranick

National Institute of Standards and Technology, Gaithersburg, Maryland 20899

J. Biomed. Opt. 16(2), 021107 (February 09, 2011). doi:10.1117/1.3533310
History: Received January 01, 2010; Revised September 13, 2010; Accepted September 15, 2010; Published February 09, 2011; Online February 09, 2011
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We experimentally compare the performance and image contrast of spontaneous Raman and coherent Stokes Raman scattering microscopy. We demonstrate the differences between these techniques on a series of geometry-controlled samples that range in complexity from a point (array of tips) to one-dimensional (line grating) and, lastly, two-dimensional (checkereboard) microstructure. Through the use of this sample series, a comparison of the focal volume, achievable signal-to-noise, and resulting image contrast is made. The results demonstrate the effciency and spatial resolution attainable in coherent Raman microscopy relative to spontaneous Raman microscopy. Additionally, we detail potential complications in the interpretation of coherent Raman images of sample fine structure, where contrast is no longer based solely on oscillator concentration but can be influenced by sample microstructure.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Hyunmin Kim ; Chris A. Michaels ; Garnett W. Bryant and Stephan J. Stranick
"Comparison of the sensitivity and image contrast in spontaneous Raman and coherent Stokes Raman scattering microscopy of geometry-controlled samples", J. Biomed. Opt. 16(2), 021107 (February 09, 2011). ; http://dx.doi.org/10.1117/1.3533310


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