Special Section on Coherent Raman Imaging Techniques and Biomedical Applications

Chemical imaging and microspectroscopy with spectral focusing coherent anti-Stokes Raman scattering

[+] Author Affiliations
Bi-Chang Chen, Jiha Sung, Xiaoxi Wu, Sang-Hyun Lim

University of Texas at Austin, Department of Chemistry and Biochemistry, 1 University Station A5300, Austin, Texas 78712

J. Biomed. Opt. 16(2), 021112 (February 15, 2011). doi:10.1117/1.3533315
History: Received June 01, 2010; Accepted October 26, 2010; Revised October 25, 2010; Published February 15, 2011; Online February 15, 2011
Text Size: A A A

We demonstrate two different coherent anti-Stokes Raman scattering (CARS) microscopy and microspectroscopy methods based on the spectral focusing mechanism. The first method uses strongly chirped broadband pulses from a single Ti:sapphire laser and generates CARS signals at the fingerprint region. Fast modulation of the time delay between the pump and Stokes laser pulses coupled with lock-in signal detection significantly reduces the nonresonant background and produces Raman-like CARS signals with a spectral resolution of 20 cm−1. The second method generates CARS signals in the CH (carbon-hydrogen) stretching region with IR supercontinuum pulses from a photonic crystal fiber. The spectral resolution of 30 cm−1 is achieved. Maximum entropy method is used to retrieve a Raman-equivalent CARS spectrum from lipid membranes. Chemical imaging and microspectroscopy are demonstrated with various samples.

Figures in this Article
© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Bi-Chang Chen ; Jiha Sung ; Xiaoxi Wu and Sang-Hyun Lim
"Chemical imaging and microspectroscopy with spectral focusing coherent anti-Stokes Raman scattering", J. Biomed. Opt. 16(2), 021112 (February 15, 2011). ; http://dx.doi.org/10.1117/1.3533315


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.