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Special Section on Coherent Raman Imaging Techniques and Biomedical Applications

Nonlinear microscopy, infrared, and Raman microspectroscopy for brain tumor analysis

[+] Author Affiliations
Tobias Meyer, Norbert Bergner, Christoph Krafft, Denis Akimov

Institute of Photonic Technology e.V., Albert-Einstein-Straße 9, 07745 Jena, Germany

Christiane Bielecki

University Medical Center Jena, Clinic for Internal Medicine II, Department of Gastroenterology, Erlanger Allee 101, 07747 Jena, Germany

Bernd F. M. Romeike

University Medical Center Jena, Department of Neuropathology, Erlanger Allee 101, 07747 Jena, Germany

Rupert Reichart, Rolf Kalff

University Medical Center Jena, Clinic for Neurosurgery, Erlanger Allee 101, 07747 Jena, Germany

Benjamin Dietzek, Jürgen Popp

Institute of Photonic Technology e.V., Albert-Einstein-Straße 9, 07745 Jena, Germany

Friedrich-Schiller-University Jena, Institute of Physical Chemistry, Helmholtzweg 4, 07743 Jena, Germany

J. Biomed. Opt. 16(2), 021113 (February 10, 2011). doi:10.1117/1.3533268
History: Received June 24, 2010; Revised November 01, 2010; Accepted November 22, 2010; Published February 10, 2011
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Contemporary brain tumor research focuses on two challenges: First, tumor typing and grading by analyzing excised tissue is of utmost importance for choosing a therapy. Second, for prognostication the tumor has to be removed as completely as possible. Nowadays, histopathology of excised tissue using haematoxylin-eosine staining is the gold standard for the definitive diagnosis of surgical pathology specimens. However, it is neither applicable in vivo, nor does it allow for precise tumor typing in those cases when only nonrepresentative specimens are procured. Infrared and Raman spectroscopy allow for very precise cancer analysis due to their molecular specificity, while nonlinear microscopy is a suitable tool for rapid imaging of large tissue sections. Here, unstained samples from the brain of a domestic pig have been investigated by a multimodal nonlinear imaging approach combining coherent anti-Stokes Raman scattering, second harmonic generation, and two photon excited fluorescence microscopy. Furthermore, a brain tumor specimen was additionally analyzed by linear Raman and Fourier transform infrared imaging for a detailed assessment of the tissue types that is required for classification and to validate the multimodal imaging approach. Hence label-free vibrational microspectroscopic imaging is a promising tool for fast and precise in vivo diagnostics of brain tumors.

Figures in this Article
© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Tobias Meyer ; Norbert Bergner ; Christiane Bielecki ; Christoph Krafft ; Denis Akimov, et al.
"Nonlinear microscopy, infrared, and Raman microspectroscopy for brain tumor analysis", J. Biomed. Opt. 16(2), 021113 (February 10, 2011). ; http://dx.doi.org/10.1117/1.3533268


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