Special Section on Coherent Raman Imaging Techniques and Biomedical Applications

Improving sensitivity in nonlinear Raman microspectroscopy imaging and sensing

[+] Author Affiliations
Rajan Arora, Georgi I. Petrov, Vladislav V. Yakovlev

University of Wisconsin – Milwaukee, Department of Physics, 1900 E. Kenwood Boulevard, Milwaukee, Wisconsin 53211

Jian Liu

PolarOnyx, Inc., 2526 Qume Drive, Suites 17–18, San Jose, California 95131

J. Biomed. Opt. 16(2), 021114 (February 17, 2011). doi:10.1117/1.3533317
History: Received June 18, 2010; Revised November 25, 2010; Accepted December 07, 2010; Published February 17, 2011; Online February 17, 2011
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Nonlinear Raman microspectroscopy based on a broadband coherent anti-Stokes Raman scattering is an emerging technique for noninvasive, chemically specific, microscopic analysis of tissues and large population of cells and particles. The sensitivity of this imaging is a critical aspect of a number of the proposed biomedical application. It is shown that the incident laser power is the major parameter controlling this sensitivity. By careful optimizing the laser system, the high-quality vibrational spectra acquisition at the multi-kHz rate becomes feasible.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Rajan Arora ; Georgi I. Petrov ; Jian Liu and Vladislav V. Yakovlev
"Improving sensitivity in nonlinear Raman microspectroscopy imaging and sensing", J. Biomed. Opt. 16(2), 021114 (February 17, 2011). ; http://dx.doi.org/10.1117/1.3533317


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