Special Section on Coherent Raman Imaging Techniques and Biomedical Applications

Hyperspectral coherent anti-Stokes Raman scattering microscopy imaging through turbid medium

[+] Author Affiliations
Rajan Arora, Georgi I. Petrov, Vladislav V. Yakovlev

University of Wisconsin – Milwaukee, Department of Physics, 1900 East Kenwood Boulevard, Milwaukee, Wisconsin 53211

J. Biomed. Opt. 16(2), 021116 (February 10, 2011). doi:10.1117/1.3541796
History: Received July 09, 2010; Revised November 25, 2010; Accepted December 16, 2010; Published February 10, 2011
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Coherent Raman microspectroscopy imaging is an emerging technique for noninvasive, chemically specific optical imaging, which can be potentially used to analyze the chemical composition and its distribution in biological tissues. In this report, a hierarchical cluster analysis was applied to hyperspectral coherent anti-Stokes Raman imaging of different chemical species through a turbid medium. It was demonstrated that by using readily available commercial software (Cytospec, Inc.) and cluster analysis, distinct chemical species can be imaged and identified through a rather thick layer of scattering medium. Once the clusters of different chemical composition were distinguished, a phase retrieval algorithm was used to convert coherent anti-Stokes Raman spectra to Raman spectra, which were used for chemical identification of hidden microscopic objects. In particular, applications to remote optical sensing of potential biological threats and to imaging through a layer of skin tissue were successfully demonstrated.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Rajan Arora ; Georgi I. Petrov and Vladislav V. Yakovlev
"Hyperspectral coherent anti-Stokes Raman scattering microscopy imaging through turbid medium", J. Biomed. Opt. 16(2), 021116 (February 10, 2011). ; http://dx.doi.org/10.1117/1.3541796


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