Research Papers: General

Transmission of polarized light in skeletal muscle

[+] Author Affiliations
Ali Shuaib, Xin Li, Gang Yao

University of Missouri, Department of Biological Engineering, Columbia, Missouri 65211

J. Biomed. Opt. 16(2), 025001 (February 16, 2011). doi:10.1117/1.3536512
History: Received March 10, 2010; Revised September 27, 2010; Accepted December 07, 2010; Published February 16, 2011; Online February 16, 2011
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Experiments were conducted to study polarized light transmission in fresh bovine skeletal muscle of varying thicknesses. Two-dimensional polarization-sensitive transmission images were acquired and analyzed using a numerical parametric fitting algorithm. The total transmittance intensity and degree-of-polarization were calculated for both central ballistic and surrounding scattering regions. Full Mueller matrix images were derived from the raw polarization images and the polar decomposition algorithm was applied to extract polarization parameters. The results suggest that polarized light propagation through skeletal muscle is affected by strong birefringence, diattenuation, multiple scattering induced depolarization and the sarcomere diffraction effect.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Ali Shuaib ; Xin Li and Gang Yao
"Transmission of polarized light in skeletal muscle", J. Biomed. Opt. 16(2), 025001 (February 16, 2011). ; http://dx.doi.org/10.1117/1.3536512


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