Research Papers: Imaging

Swept-source optical coherence tomography of lower limb wound healing with histopathological correlation

[+] Author Affiliations
Ananya Barui, Rusha Patra, Raunak Kumar Das, Santanu Dhara, Pranab K Dutta, Jyotirmoy Chatterjee

IIT Kharagpur, School of Medical Science and Technology, Kharagpur, West Bengal 721302 India

Provas Banerjee

Banerjee's Biomedical Research Foundation, 700 005 India

J. Biomed. Opt. 16(2), 026010 (February 01, 2011). doi:10.1117/1.3535593
History: Received June 21, 2010; Revised December 06, 2010; Accepted December 17, 2010; Published February 01, 2011; Online February 01, 2011
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Direct noninvasive visualization of wound bed with depth information is important to understand the tissue repair. We correlate skin swept-source–optical coherence tomography (OCT) with histopathological and immunohistochemical evaluation on traumatic lower limb wounds under honey dressing to compare and assess the tissue repair features acquired noninvasively and invasively. Analysis of optical biopsy identifies an uppermost brighter band for stratum corneum with region specific thickness (p < 0.0001) and gray-level intensity (p < 0.0001) variation. Below the stratum corneum, variation in optical intensities is remarkable in different regions of the wound bed. Correlation between OCT and microscopic observations are explored especially in respect to progressive growth and maturation of the epithelial and subepithelial components. Characteristic transition of uniform hypolucid band in OCT image for depigmented zone to wavy highly lucid band in the pigmented zone could be directly correlated with the microscopic findings. The transformation of prematured epithelium of depigmented area, with low expression of E-cadherin, to matured epithelium with higher E-cadherin expression in pigmented zone, implicated plausible change in their optical properties as depicted in OCT. This correlated evaluation of multimodal images demonstrates applicability of swept-source-OCT in wound research and importance of integrated approach in validation of new technology.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Ananya Barui ; Provas Banerjee ; Rusha Patra ; Raunak Kumar Das ; Santanu Dhara, et al.
"Swept-source optical coherence tomography of lower limb wound healing with histopathological correlation", J. Biomed. Opt. 16(2), 026010 (February 01, 2011). ; http://dx.doi.org/10.1117/1.3535593


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