Research Papers: Imaging

Hyperspectral imaging of atherosclerotic plaques in vitro

[+] Author Affiliations
Eivind L. P. Larsen, Lise L. Randeberg, Astrid Aksnes, Lars O. Svaasand

Norwegian University of Science and Technology, Department of Electronics and Telecommunications, 7034 Trondheim, Norway

Elisabeth Olstad

University Hospital of North Norway, Department of Radiology, Division of Diagnostic Services, 9038 Tromsø, Norway

Olav A. Haugen

Children's and Women's Health, Norwegian University of Science and Technology, Department of Laboratory Medicine, 7006 Trondheim, Norway

J. Biomed. Opt. 16(2), 026011 (February 22, 2011). doi:10.1117/1.3540657
History: Received April 21, 2010; Revised December 21, 2010; Accepted December 21, 2010; Published February 22, 2011; Online February 22, 2011
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Vulnerable plaques constitute a risk for serious heart problems, and are difficult to identify using existing methods. Hyperspectral imaging combines spectral- and spatial information, providing new possibilities for precise optical characterization of atherosclerotic lesions. Hyperspectral data were collected from excised aorta samples (n = 11) using both white-light and ultraviolet illumination. Single lesions (n = 42) were chosen for further investigation, and classified according to histological findings. The corresponding hyperspectral images were characterized using statistical image analysis tools (minimum noise fraction, K-means clustering, principal component analysis) and evaluation of reflectance/fluorescence spectra. Image analysis combined with histology revealed the complexity and heterogeneity of aortic plaques. Plaque features such as lipids and calcifications could be identified from the hyperspectral images. Most of the advanced lesions had a central region surrounded by an outer rim or shoulder-region of the plaque, which is considered a weak spot in vulnerable lesions. These features could be identified in both the white-light and fluorescence data. Hyperspectral imaging was shown to be a promising tool for detection and characterization of advanced atherosclerotic plaques in vitro. Hyperspectral imaging provides more diagnostic information about the heterogeneity of the lesions than conventional single point spectroscopic measurements.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Eivind L. P. Larsen ; Lise L. Randeberg ; Elisabeth Olstad ; Olav A. Haugen ; Astrid Aksnes, et al.
"Hyperspectral imaging of atherosclerotic plaques in vitro", J. Biomed. Opt. 16(2), 026011 (February 22, 2011). ; http://dx.doi.org/10.1117/1.3540657


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