Research Papers: Imaging

Noninvasive optical coherence tomography monitoring of structure and hydration changes of human corneas in different preservation media

[+] Author Affiliations
Yicong Wu, Xingde Li

University of Washington, Department of Bioengineering, Seattle, Washington 98195

Johns Hopkins University, Department of Biomedical Engineering, Baltimore, Maryland 21205

Dominic Clarke, Aby Mathew

BioLife Solutions, Inc., Bothell, Washington 98021

Ian Nicoud

Fred Hutchinson Cancer Research Center, Seattle, Washington 98109

J. Biomed. Opt. 16(2), 026015 (February 02, 2011). doi:10.1117/1.3541792
History: Received May 28, 2010; Revised November 20, 2010; Accepted December 23, 2011; Published February 02, 2011; Online February 02, 2011
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The influence of different tissue preservation (a test solution under development and a standard storage solution) on human cornea morphology, refractive index and hydration was assessed noninvasively by ultrahigh-resolution optical coherence tomography (OCT) over time. For 28 days’ or 15 days’ storage in the preservation media, corneas in the two media exhibited different structural changes with different onset times including epithelial desquamation, edema-induced cornea thickening and change in tissue refractive index. It was found that the variation of the group refractive index over time was only about 2%, while 25% variation of hydration was observed in the storage and subsequent return to normothermic conditions in both preservation media. The results suggest the two media involved different but correlated preservation mechanisms. This study demonstrates that the noncontact, noninvasive, and high-resolution OCT is a powerful tool for noninvasive characterization of tissue morphological changes and hydration process and for assessment of the effects of preservation media on stored tissue integrity. © 2011 Society of Photo-Optical Instrumentation Engineers.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Yicong Wu ; Dominic Clarke ; Aby Mathew ; Ian Nicoud and Xingde Li
"Noninvasive optical coherence tomography monitoring of structure and hydration changes of human corneas in different preservation media", J. Biomed. Opt. 16(2), 026015 (February 02, 2011). ; http://dx.doi.org/10.1117/1.3541792


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