Research Papers: Therapeutic

Tissue responses to hexyl 5-aminolevulinate-induced photodynamic treatment in syngeneic orthotopic rat bladder cancer model: possible pathways of action

[+] Author Affiliations
Carl-Jørgen Arum

St. Olavs University Hospital Trondheim, Department of Surgery, N-7006 Trondheim, Norway

Norwegian University of Science and Technology, Department of Cancer Research and Molecular Medicine, N-7006 Trondheim, Norway

Odrun A. Gederaas, Hans E. Krokan, Duan Chen, Chun-Mei Zhao

Norwegian University of Science and Technology, Department of Cancer Research and Molecular Medicine, N-7006 Trondheim, Norway

Eivind L. P. Larsen, Lise L. Randeberg, Lars O. Svaasand

Norwegian University of Science and Technology, Department of Electronics and Telecommunications, N-7034 Trondheim, Norway

Astrid Hjelde

Norwegian University of Science and Technology, Department of Circulation and Medical Imaging, N-7006 Trondheim, Norway

J. Biomed. Opt. 16(2), 028001 (February 09, 2011). doi:10.1117/1.3536536
History: Received September 08, 2010; Revised November 14, 2010; Accepted December 07, 2010; Published February 09, 2011; Online February 09, 2011
Text Size: A A A

Orthotopic bladder cancer model in rats mimics human bladder cancer with respect to urothelial tumorigenesis and progression. Utilizing this model at pT1 (superficial stage), we analyze the tissue responses to hexyl 5-aminolevulinate-induced photodynamic therapy (HAL-PDT). In comparison to untreated rats, HAL-PDT causes little change in tumor-free rat bladder but induces inflammatory changes with increased lymphocytes and mononuclear cell infiltration in rat bladders with tumor. Immunohistochemistry reveals that HAL-PDT is without effect on proliferating cell nuclear antigen expression within the tumor and increases caspase-3 expression in both normal urothelium and the tumor. Transmission electron microscopy reveals severe mitochondrial damage, formations of apoptotic bodies, vacuoles, and lipofuscin bodies, but no microvillus-formed niches in HAL-PDT-treated bladder cancer rats. Bioinformatics analysis of the gene expression profile indicates an activation of T-cell receptor signaling pathway in bladder cancer rats without PDT. HAL-PDT increases the expression of CD3 and CD45RA in the tumor (determined by immunohistochemistry). We suggest that pathways of action of HAL-PDT may include, at least, activations of mitochondrial apoptosis and autophagy, breakdown of cancer stem cell niches, and importantly, enhancement of T-cell activation.

Figures in this Article
© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Carl-Jørgen Arum ; Odrun A. Gederaas ; Eivind L. P. Larsen ; Lise L. Randeberg ; Astrid Hjelde, et al.
"Tissue responses to hexyl 5-aminolevulinate-induced photodynamic treatment in syngeneic orthotopic rat bladder cancer model: possible pathways of action", J. Biomed. Opt. 16(2), 028001 (February 09, 2011). ; http://dx.doi.org/10.1117/1.3536536


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.