Research Papers: Therapeutic

Optical transfection using an endoscope-like system

[+] Author Affiliations
Nan Ma, Kishan Dholakia

University of St. Andrews, School of Physics & Astronomy, St. Andrews, Fife KY16 9SS United Kingdom

Frank Gunn-Moore

University of St. Andrews, School of Biology, Medical and Biological Sciences Building, St. Andrews, KY16 9TF United Kingdom

J. Biomed. Opt. 16(2), 028002 (February 15, 2011). doi:10.1117/1.3541781
History: Received December 15, 2010; Accepted December 16, 2010; Published February 15, 2011; Online February 15, 2011
Text Size: A A A

Optical transfection is a powerful method for targeted delivery of therapeutic agents to biological cells. A tightly focused pulsed laser beam may transiently change the permeability of a cell membrane to facilitate the delivery of foreign genetic material into cells. We report the first realization of an endoscope-like integrated system for optical transfection. An imaging fiber (coherent optical fiber bundle) with ∼6000 cores (pixels) embedded in a fiber cladding of ∼300 μm in diameter, produces an image circle (area) of ∼270 μm diam. This imaging fiber, with an ordered axicon lens array chemically etched at its exit face, is used for the delivery of a femtosecond laser to the cell membrane for optical transfection along with subcellular resolution imaging. A microcapillary-based microfluidic system for localized drug delivery was also combined in this miniature, flexible system. Using this novel system, a plasmid transfection efficiency up to ∼72% was obtained for CHO-K1 cells. This endoscope-like system opens a range of exciting applications, in particular, in the targeted in vivo optical microsurgery area.

Figures in this Article
© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Nan Ma ; Frank Gunn-Moore and Kishan Dholakia
"Optical transfection using an endoscope-like system", J. Biomed. Opt. 16(2), 028002 (February 15, 2011). ; http://dx.doi.org/10.1117/1.3541781


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.