Research Papers: General

Kinetics of carotenoid distribution in human skin in vivo after exogenous stress: disinfectant and wIRA-induced carotenoid depletion recovers from outside to inside

[+] Author Affiliations
Joachim W. Fluhr, Heike Richter, Wolfram Sterry, Juergen Lademann, Maxim E. Darvin

Charité–Universitätsmedizin, Center of Experimental Applied Cutaneous Physiology, Department of Dermatology and Allergology, D-10117 Berlin, Germany

Peter Caspers, J. Andre van der Pol

River Diagnostics B.V., 3015 GE, Rotterdam, Netherlands

J. Biomed. Opt. 16(3), 035002 (March 16, 2011). doi:10.1117/1.3555183
History: Received November 04, 2010; Revised January 24, 2011; Accepted January 26, 2011; Published March 16, 2011; Online March 16, 2011
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The human organism has developed a protection system against the destructive effect of free radicals. The aim of the present study was to investigate the extent of exogenous stress factors such as disinfectant and IR-A radiation on the skin, and their influence on the kinetics of carotenoids distribution during the recovery process. Ten healthy volunteers were assessed with resonance spectroscopy using an Argon-laser at 488 nm to excite the carotenoids in vivo. Additionally, Raman-confocal-micro-spectroscopy measurements were performed using a model 3510 Skin Composition Analyzer with spatially resolved measurements down to 30 μm. The measurements were performed at a baseline of 20, 40, 60, and 120 min after an external stressor consisting either of water-filtered infrared A (wIRA) with 150 mW/cm2 or 1 ml/cm2 of an alcoholic disinfectant. Both Raman methods were capable to detect the infrared-induced depletion of carotenoids. Only Raman-microspectroscopy could reveal the carotenoids decrease after topical disinfectant application. The carotenoid-depletion started at the surface. After 60 min, recovery starts at the surface while deeper parts were still depleted. The disinfectant- and wIRA-induced carotenoid depletion in the epidermis recovers from outside to inside and probably delivered by sweat and sebaceous glands. We could show that the Raman microscopic spectroscopy is suited to analyze the carotenoid kinetic of stress effects and recovery.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Joachim W. Fluhr ; Peter Caspers ; J. Andre van der Pol ; Heike Richter ; Wolfram Sterry, et al.
"Kinetics of carotenoid distribution in human skin in vivo after exogenous stress: disinfectant and wIRA-induced carotenoid depletion recovers from outside to inside", J. Biomed. Opt. 16(3), 035002 (March 16, 2011). ; http://dx.doi.org/10.1117/1.3555183


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