Research Papers: Imaging

Deep in vivo two-photon imaging of blood vessels with a new dye encapsulated in pluronic nanomicelles

[+] Author Affiliations
Mathieu Maurin, Olivier Stéphan, Jean-Claude Vial

Laboratoire de Spectrométrie Physique, CNRS UMR 5588, 140 Avenue de la Physique, BP 87, 38402, Saint Martin d’Hères, France

Seth R. Marder

Georgia Institute of Technology, School of Chemistry and Biochemistry, and Center for Organic Electronics and Photonics, Atlantic Blvd., Atlanta, Georgia 30332–0400

Boudewijn van der Sanden

Institut des Neurosciences Grenoble, INSERM U836, Chemin Fortuné Ferrini, BP 170, 38042, La Tronche, Grenoble, 38042 France

J. Biomed. Opt. 16(3), 036001 (March 01, 2011). doi:10.1117/1.3548879
History: Received September 14, 2010; Revised December 17, 2010; Accepted January 06, 2011; Published March 01, 2011; Online March 01, 2011
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Our purpose is to test if Pluronic® fluorescent nanomicelles can be used for in vivo two-photon imaging of both the normal and the tumor vasculature. The nanomicelles were obtained after encapsulating a hydrophobic two-photon dye: di-stryl benzene derivative, in Pluronic block copolymers. Their performance with respect to imaging depth, blood plasma staining, and diffusion across the tumor vascular endothelium is compared to a classic blood pool dye Rhodamin B dextran (70 kDa) using two-photon microscopy. Pluronic nanomicelles show, like Rhodamin B dextran, a homogeneous blood plasma staining for at least 1 h after intravenous injection. Their two-photon imaging depth is similar in normal mouse brain, using 10 times less injected mass. In contrast with Rhodamin B dextran, no extravasation is observed in leaky tumor vessels due to their large size: 20–100 nm. In conclusion, Pluronic nanomicelles can be used as a blood pool dye, even in leaky tumor vessels. The use of Pluronic block copolymers is a valuable approach for encapsulating two-photon fluorescent dyes that are hydrophobic and not suitable for intravenous injection.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Mathieu Maurin ; Olivier Stéphan ; Jean-Claude Vial ; Seth R. Marder and Boudewijn van der Sanden
"Deep in vivo two-photon imaging of blood vessels with a new dye encapsulated in pluronic nanomicelles", J. Biomed. Opt. 16(3), 036001 (March 01, 2011). ; http://dx.doi.org/10.1117/1.3548879


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