Research Papers: Imaging

Dynamic analysis of pathogen-infected host cells using quantitative phase microscopy

[+] Author Affiliations
Seungrag Lee, Ji Yong Lee, Chang-Soo Park

Gwangju Institute of Science and Technology, Department of Information and Communications, 1 Oryong-dong, Buk-gu, Gwangju, 500–712 Republic of Korea

Young Ran Kim

Dongshin University, Department of Oriental Medicine Materials, 252 Daeho-dong, Naju, Jeonnam, 520–714 Republic of Korea

Joon Haeng Rhee

Chonnam National University Medical School, Clinical Vaccine R&D Center, Research Institute of Vibrio Infection and Genome Research Center for Enteropathogenic Bacteria, Republic of Korea

Dug Young Kim

Yonsei University, Department of Physics, 262 Seongsanno, Seodaemun-gu, Seoul, 120–749 Republic of Korea

J. Biomed. Opt. 16(3), 036004 (March 16, 2011). doi:10.1117/1.3548882
History: Received June 23, 2010; Revised November 24, 2010; Accepted January 07, 2011; Published March 16, 2011; Online March 16, 2011
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We present the real-time quantitative analysis of Vibrio vulnificus–infected host cells using quantitative phase microscopy (QPM) based on interferometric techniques. This provides the ability to retrieve the phase or optical path-length distribution over the cell with nanometer path-length sensitivity from a single interferogram image. We have used QPM to study dynamic cell morphologic changes and to noninvasively quantify the cell volumes of rat basophilic leukemia RBL-2H3 cells infected with V. vulnificus strains: wild type (MO6–24/O) and RtxA1 toxin mutant (CMM770). During the process of V. vulnificus infection in RBL-2H3 cells, the dynamic changes of quantitative phase images, cell volumes, and areas were observed in real time using QPM. In contrast, dramatic changes were not detected in RBL-2H3 cells infected with the noncytotoxic RtxA1 toxin mutant. The results showed good correlation between QPM analysis and biochemical assays, such as lactate dehydrogenase assay or β-hexosaminidase release assay. We suggest that QPM is a powerful quantitative method to study the dynamic process of host cells infected with pathogens in a noninvasive manner.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Seungrag Lee ; Young Ran Kim ; Ji Yong Lee ; Joon Haeng Rhee ; Chang-Soo Park, et al.
"Dynamic analysis of pathogen-infected host cells using quantitative phase microscopy", J. Biomed. Opt. 16(3), 036004 (March 16, 2011). ; http://dx.doi.org/10.1117/1.3548882


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