Research Papers: General

Toward label-free Raman-activated cell sorting of cardiomyocytes derived from human embryonic stem cells

[+] Author Affiliations
Flavius C. Pascut, Ioan Notingher

University of Nottingham, School of Physics and Astronomy, Nottingham NG7 2RD, United Kingdom

Huey T. Goh, Vinoj George, Chris Denning

University of Nottingham, Wolfson Centre for Stem Cells, Tissue Engineering and Modelling, Centre for Biomolecular Sciences, University Park, Nottingham NG7 2RD, United Kingdom

J. Biomed. Opt. 16(4), 045002 (April 21, 2011). doi:10.1117/1.3570302
History: Received January 20, 2011; Revised March 02, 2011; Accepted March 04, 2011; Published April 21, 2011; Online April 21, 2011
Text Size: A A A

Raman micro-spectroscopy (RMS) has been recently proposed for label-free phenotypic identification of human embryonic stem cells (hESC)-derived cardiomyocytes. However, the methods used for measuring the Raman spectra led to acquisition times of minutes per cell, which is prohibitive for rapid cell sorting applications. In this study we evaluated two measurement strategies that could reduce the measurement time by a factor of more than 100. We show that sampling individual cells with a laser beam focused to a line could eliminate the need of cell raster scanning and achieve high prediction accuracies (>95% specificity and >96% sensitivity) with acquisition times ∼5 seconds per cell. However, the use of commercially-available higher power lasers could potentially lead to sorting speeds of ∼10 cells per s. This would start to progress RMS to the field of cell sorting for applications such as enrichment and purification of hESC-derived cardiomyocytes.

Figures in this Article
© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Flavius C. Pascut ; Huey T. Goh ; Vinoj George ; Chris Denning and Ioan Notingher
"Toward label-free Raman-activated cell sorting of cardiomyocytes derived from human embryonic stem cells", J. Biomed. Opt. 16(4), 045002 (April 21, 2011). ; http://dx.doi.org/10.1117/1.3570302


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

Advertisement


 

  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.