Research Papers: Imaging

Optical coherence tomography for high-resolution imaging of mouse development in utero

[+] Author Affiliations
Saba H. Syed

University of Houston, Department of Biomedical Engineering, 4800 Calhoun Road, 3605 Cullen Boulevard, Houston, Texas 77204

Kirill V. Larin

University of Houston, Department of Biomedical Engineering, 4800 Calhoun Road, 3605 Cullen Boulevard, Houston, Texas 77204

Baylor College of Medicine, Department of Molecular Physiology and Biophysics, One Baylor Plaza, Houston, Texas 77030

Mary E. Dickinson, Irina V. Larina

Baylor College of Medicine, Department of Molecular Physiology and Biophysics, One Baylor Plaza, Houston, Texas 77030

J. Biomed. Opt. 16(4), 046004 (April 01, 2011). doi:10.1117/1.3560300
History: Revised February 01, 2011; Received October 26, 2010; Accepted February 09, 2011; Published April 01, 2011; Online April 01, 2011
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Although the mouse is a superior model to study mammalian embryonic development, high-resolution live dynamic visualization of mouse embryos remain a technical challenge. We present optical coherence tomography as a novel methodology for live imaging of mouse embryos through the uterine wall thereby allowing for time lapse analysis of developmental processes and direct phenotypic analysis of developing embryos. We assessed the capability of the proposed methodology to visualize structures of the living embryo from embryonic stages 12.5 to 18.5 days postcoitus. Repetitive in utero embryonic imaging is demonstrated. Our work opens the door for a wide range of live, in utero embryonic studies to screen for mutations and understand the effects of pharmacological and toxicological agents leading to birth defects.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Saba H. Syed ; Kirill V. Larin ; Mary E. Dickinson and Irina V. Larina
"Optical coherence tomography for high-resolution imaging of mouse development in utero", J. Biomed. Opt. 16(4), 046004 (April 01, 2011). ; http://dx.doi.org/10.1117/1.3560300


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