Research Papers: Imaging

Reconstruction of fluorescence distribution hidden in biological tissue using mesoscopic epifluorescence tomography

[+] Author Affiliations
Saskia Björn, Karl-Hans Englmeier, Vasilis Ntziachristos, Ralf Schulz

Institute for Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany

J. Biomed. Opt. 16(4), 046005 (April 04, 2011). doi:10.1117/1.3560631
History: Received October 19, 2010; Revised February 08, 2011; Accepted February 10, 2011; Published April 04, 2011; Online April 04, 2011
Text Size: A A A

Mesoscopic epifluorescence tomography is a novel technique that discovers fluorescence bio-distribution in small animals by tomographic means in reflectance geometry. A collimated laser beam is scanned over the skin surface to excite fluorophores hidden within the tissue while a CCD camera acquires an image of the fluorescence emission for each source position. This configuration is highly efficient in the visible spectrum range where trans-illumination imaging of small animals is not feasible due to the high tissue absorption and scattering in biological organisms. The reconstruction algorithm is similar to the one used in fluorescence molecular tomography. However, diffusion theory cannot be employed since the source-detector separation for most image pixels is comparable to or below the scattering length of the tissue. Instead Monte Carlo simulations are utilized to predict the sensitivity functions. In a phantom study we show the effect of using enhanced source grid arrangements during the data acquisition and the reconstruction process to minimize boundary artefacts. Furthermore, we present ex vivo data that show high spatial resolution and quantitative accuracy in heterogeneous tissues using GFP-like fluorescence in B6-albino mice up to a depth of 1100 μm.

Figures in this Article
© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Saskia Björn ; Karl-Hans Englmeier ; Vasilis Ntziachristos and Ralf Schulz
"Reconstruction of fluorescence distribution hidden in biological tissue using mesoscopic epifluorescence tomography", J. Biomed. Opt. 16(4), 046005 (April 04, 2011). ; http://dx.doi.org/10.1117/1.3560631


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.