Research Papers: Imaging

Long-term imaging of mouse embryos using adaptive harmonic generation microscopy

[+] Author Affiliations
Anisha Thayil, Alexander Jesacher, Tony Wilson, Martin Booth

University of Oxford, Department of Engineering Science, Parks Road, Oxford, Oxfordshire OX1 3PJ, United Kingdom

Tomoko Watanabe, Shankar Srinivas

University of Oxford, Department of Physiology Anatomy and Genetics, Oxford, Oxfordshire OX1 3QX, United Kingdom

J. Biomed. Opt. 16(4), 046018 (April 28, 2011). doi:10.1117/1.3569614
History: Received November 12, 2011; Accepted March 02, 2011; Revised February 22, 2011; Published April 28, 2011; Online April 28, 2011
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We present a detailed description of an adaptive harmonic generation (HG) microscope and culture techniques that permit long-term, three-dimensional imaging of mouse embryos. HG signal from both pre- and postimplantation stage (0.5–5.5 day-old) mouse embryos are fully characterized. The second HG images reveal central spindles during cytokinesis whereas third HG images show several features, such as lipid droplets, nucleoli, and plasma membranes. The embryos are found to develop normally during one-day-long discontinuous HG imaging, permitting the observation of several dynamic events, such as morula compaction and blastocyst formation.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Anisha Thayil ; Tomoko Watanabe ; Alexander Jesacher ; Tony Wilson ; Shankar Srinivas, et al.
"Long-term imaging of mouse embryos using adaptive harmonic generation microscopy", J. Biomed. Opt. 16(4), 046018 (April 28, 2011). ; http://dx.doi.org/10.1117/1.3569614


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