Research Papers: Imaging

Cell tracking in live Caenorhabditis elegans embryos via third harmonic generation imaging microscopy measurements

[+] Author Affiliations
George J. Tserevelakis, Costas Fotakis

Foundation of Research and Technology-Hellas, Institute of Electronic Structure and Laser, P.O. Box 1385, Heraklion, Crete, 71110, Greece

University of Crete, Department of Physics, 71110, Heraklion, Crete, Greece

George Filippidis

Foundation of Research and Technology-Hellas, Institute of Electronic Structure and Laser, P.O. Box 1385, Heraklion, Crete, 71110, Greece

Evgenia V. Megalou, Nektarios Tavernarakis

Foundation of Research and Technology, Institute of Molecular Biology and Biotechnology, Heraklion, Crete, 71110, Greece

J. Biomed. Opt. 16(4), 046019 (April 20, 2011). doi:10.1117/1.3569615
History: Received November 12, 2010; Revised January 20, 2011; Accepted March 02, 2011; Published April 20, 2011; Online April 20, 2011
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In this study, we demonstrate the potential of employing third harmonic generation (THG) imaging microscopy measurements for cell tracking studies in live Caenorhabditis elegans (C. elegans) embryos. A 1028-nm femtosecond laser was used for the excitation of unstained C. elegans samples. Different C. elegans embryonic stages (from two-cell to threefold) were imaged. Live biological specimens were irradiated for prolonged periods of time (up to 7 h), testifying to the nondestructive nature of this nonlinear imaging technique. Thus, THG image contrast modality is a powerful diagnostic tool for probing in vivo cell division during early embryogenesis.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

George J. Tserevelakis ; George Filippidis ; Evgenia V. Megalou ; Costas Fotakis and Nektarios Tavernarakis
"Cell tracking in live Caenorhabditis elegans embryos via third harmonic generation imaging microscopy measurements", J. Biomed. Opt. 16(4), 046019 (April 20, 2011). ; http://dx.doi.org/10.1117/1.3569615


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