Research Papers: Imaging

Waveguide evanescent field fluorescence microscopy: high contrast imaging of a domain forming mixed lipid Langmuir-Blodgett monolayer mimicking lung surfactant

[+] Author Affiliations
Abdollah Hassanzadeh, Silvia Mittler

University of Western Ontario, Department of Physics and Astronomy, London, Ontario, N6A 3K7 Canada

J. Biomed. Opt. 16(4), 046022 (April 29, 2011). doi:10.1117/1.3569095
History: Received November 16, 2010; Revised February 25, 2011; Accepted February 28, 2011; Published April 29, 2011; May 06, 2011; Online April 29, 2011
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Waveguide evanescent field fluorescence (WEFF) microscopy is a new development that allows the imaging of contact regions between biological cells and their substratum, as well as imaging of ultrathin films such as monomolecular Langmuir–Blodgett (LB) films. Mixed-lipid monolayer systems mimicking lung surfactant were fabricated on waveguides using the LB technique and imaged by both WEFF and standard wide field epi-fluorescence microscopy. These two technologies were compared with respect to contrast, photobleaching, and sensitivity. It was found that WEFF microscopy produced images with a much higher contrast, lower photobleaching, and higher sensitivity. In addition, fine structures in the lipidic domains were observed for the first time.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Abdollah Hassanzadeh and Silvia Mittler
"Waveguide evanescent field fluorescence microscopy: high contrast imaging of a domain forming mixed lipid Langmuir-Blodgett monolayer mimicking lung surfactant", J. Biomed. Opt. 16(4), 046022 (April 29, 2011). ; http://dx.doi.org/10.1117/1.3569095


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