Research Papers: Therapeutic

Effect of 405-nm high-intensity narrow-spectrum light on fibroblast-populated collagen lattices: an in vitro model of wound healing

[+] Author Affiliations
Richard McDonald, M. Helen Grant

University of Strathclyde, Bioengineering Unit, Wolfson Centre, 106 Rottenrow, Glasgow, Strathclyde G4 0NW United Kingdom

Scott J. MacGregor, John G. Anderson, Michelle Maclean

University of Strathclyde, The Robertson Trust Laboratory for Electronic Sterilisation Technologies, 204 George Street, Glasgow, Scotland G1 1XW United Kingdom

J. Biomed. Opt. 16(4), 048003 (April 04, 2011). doi:10.1117/1.3561903
History: Received November 16, 2010; Revised February 11, 2011; Accepted February 11, 2011; Published April 04, 2011; Online April 04, 2011
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High-intensity narrow-spectrum (HINS) 405-nm light is a novel technology developed to address the significant problem of health-care associated infection. Its potential for wound-decontamination applications is assessed on mammalian cells and bacteria. The fibroblast-populated collagen lattice (FPCL) is used as an in vitro model of wound healing, and the effect of HINS light on contraction is examined. Effects on cell proliferation, morphological changes, and α-smooth muscle actin (α-SMA) expression are investigated. Bactericidal effects are assessed using the bacterium Staphylococcus epidermidis. Low doses of HINS light were found to have no significant inhibitory effects on FPCL contraction, cell proliferation, or α-SMA expression. Doses of up to 18 Jcm−2 had no significant inhibitory effects on FPCL cell numbers, and this dose was shown to cause almost complete inactivation of bacteria. These results show that HINS light has potential for disinfection applications without adversely influencing wound healing.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Richard McDonald ; Scott J. MacGregor ; John G. Anderson ; Michelle Maclean and M. Helen Grant
"Effect of 405-nm high-intensity narrow-spectrum light on fibroblast-populated collagen lattices: an in vitro model of wound healing", J. Biomed. Opt. 16(4), 048003 (April 04, 2011). ; http://dx.doi.org/10.1117/1.3561903


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