Special Section on Photonics and Nanotechnology in Biophysics and Biomedical Research

Multiphoton microscopy study of the morphological and quantity changes of collagen and elastic fiber components in keloid disease

[+] Author Affiliations
Jianxin Chen, Shuangmu Zhuo, Xingshan Jiang, Xiaoqin Zhu, Liqin Zheng, Shusen Xie

Fujian Normal University, Institute of Laser and Optoelectronics Technology, Fujian Provincial Key Laboratory for Photonics Technology, Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of Education, Fuzhou 350007, China

Bifang Lin

Fuzhou First Hospital, Department of Dermatology, Fuzhou 350009, China

Haishan Zeng

British Columbia Cancer Agency Research Centre, Cancer Imaging Department, Vancouver, BC, V5Z 1L3, Canada

J. Biomed. Opt. 16(5), 051305 (May 02, 2011). doi:10.1117/1.3569617
History: Received November 24, 2010; Revised February 22, 2011; Accepted March 02, 2011; Published May 02, 2011; Online May 02, 2011
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Multiphoton microscopy was used to study the extracellular matrix of keloid at the molecular level without tissue fixation and staining. Direct imaging of collagen and elastin was achieved by second harmonic generation and two-photon excited fluorescence, respectively. The morphology and quantity of collagen and elastin in keloid were characterized and quantitatively analyzed in comparison to normal skin. The study demonstrated that in keloid, collagen content increased in both the upper dermis and the deep dermis, while elastin mostly showed up in the deep dermis and its quantity is higher compared to normal skin. This suggests the possibility that abnormal fibroblasts synthesized an excessive amount of collagen and elastin at the beginning of keloid formation, corresponding to the observed deep dermis, while after a certain time point, the abnormal fibroblast produced mostly collagen, corresponding to the observed upper dermis. The morphology of collagen and elastin in keloid was disrupted and presented different variations. In the deep dermis, elastic fibers showed node structure, while collagen showed obviously regular gaps between adjacent bundles. In the upper dermis, collagen bundles aligned in a preferred direction, while elastin showed as sparse irregular granules. This new molecular information provided fresh insight about the development process of keloid.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Jianxin Chen ; Shuangmu Zhuo ; Xingshan Jiang ; Xiaoqin Zhu ; Liqin Zheng, et al.
"Multiphoton microscopy study of the morphological and quantity changes of collagen and elastic fiber components in keloid disease", J. Biomed. Opt. 16(5), 051305 (May 02, 2011). ; http://dx.doi.org/10.1117/1.3569617


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