Research Papers: Imaging

Multiexposure laser speckle contrast imaging of the angiogenic microenvironment

[+] Author Affiliations
Abhishek Rege, Kartikeya Murari, Alan Seifert, Nitish V. Thakor

Johns Hopkins University School of Medicine, Department of Biomedical Engineering, 720 Rutland Avenue, Baltimore, Maryland 21205

Arvind P. Pathak

Johns Hopkins University School of Medicine, Russell H. Morgan Department of Radiology and Radiological Science, 600 North Wolfe Street, Baltimore, Maryland 21287

J. Biomed. Opt. 16(5), 056006 (May 31, 2011). doi:10.1117/1.3582334
History: Received July 21, 2010; Revised March 22, 2011; Accepted March 24, 2011; Published May 31, 2011; Online May 31, 2011
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We report the novel use of laser speckle contrast imaging (LSCI) at multiple exposure times (meLSCI) for enhanced in vivo imaging of the microvascular changes that accompany angiogenesis. LSCI is an optical imaging technique that can monitor blood vessels and the flow therein at a high spatial resolution without requiring the administration of an exogenous contrast agent. LSCI images are obtained under red (632 nm) laser illumination at seven exposure times (1–7 ms) and combined using a curve-fitting approach to obtain high-resolution meLSCI images of the rat brain vasculature. To evaluate enhancement in in vivo imaging performance, meLSCI images are statistically compared to individual LSCI images obtained at a single exposure time. We find that meLSCI reduced the observed variability in the LSCI-based blood-flow estimates by 30% and improved the contrast-to-noise ratio in regions with high microvessel density by 41%. The ability to better distinguish microvessels, makes meLSCI uniquely suited to longitudinal imaging of changes in the vascular microenvironment induced by pathological angiogenesis. We demonstrate this utility of meLSCI by sequentially monitoring, over days, the microvascular changes that accompany wound healing in a mouse ear model.

Figures in this Article
© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Topics

Brain ; Lasers ; Speckle ; Blood ; Ear

Citation

Abhishek Rege ; Kartikeya Murari ; Alan Seifert ; Arvind P. Pathak and Nitish V. Thakor
"Multiexposure laser speckle contrast imaging of the angiogenic microenvironment", J. Biomed. Opt. 16(5), 056006 (May 31, 2011). ; http://dx.doi.org/10.1117/1.3582334


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