Research Papers: Imaging

Silver nanostructure sensing platform for maximum-contrast fluorescence cell imaging

[+] Author Affiliations
Kyung-Min Lee, Arup Neogi

University of North Texas, Department of Physics, Denton, Texas 76203

Purnima Basu Neogi

University of North Texas, Department of Biology, Denton, Texas 76203

Minjung Kim, Bongsoo Kim

Department of Chemistry, KAIST, Deajeon, 305-701, Korea

Rafal Luchowski, Zygmunt Gryczynski

University of North Texas Health Science Center, Department of Molecular Biology and Immunology Fort Worth, Texas 76107

Nils Calander

Macquarie University, Department of Physics, NSW 2109, Sydney, Australia

Tae-Youl Choi

University of North Texas, Department of Mechanical and Energy Engineering, Denton, Texas 76207

J. Biomed. Opt. 16(5), 056008 (May 10, 2011). doi:10.1117/1.3579157
History: Received October 12, 2010; Revised March 06, 2011; Accepted March 25, 2011; Published May 10, 2011; Online May 10, 2011
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We present herein a silver nanostructure-assisted sensing platform which consists of a combined structure of Ag nanowire (NW) and nanodot (ND) array. Highly enhanced fluorescence from fluorophore is attributed to a strongly coupled optical near-field interaction between proximately located Ag NW and NDs. We obtained enhanced fluorescence intensity with up to 140 folds, as contrasted from background intensity, reaching a theoretical maximum value. On the other hand, fluorescence lifetime was greatly reduced to 0.27 ns (from 2.17 ns for the same fluorophores without nanostructure). This novel platform can be a promising utility for optical imaging and labeling of biological systems with a great sensitivity.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Kyung-Min Lee ; Arup Neogi ; Purnima Basu Neogi ; Minjung Kim ; Bongsoo Kim, et al.
"Silver nanostructure sensing platform for maximum-contrast fluorescence cell imaging", J. Biomed. Opt. 16(5), 056008 (May 10, 2011). ; http://dx.doi.org/10.1117/1.3579157


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