Research Papers: Sensing

Spectroscopic signature of mouse embryonic stem cell–derived hepatocytes using synchrotron Fourier transform infrared microspectroscopy

[+] Author Affiliations
Kanjana Thumanu, Waraporn Tanthanuch

Synchrotron Light Research Institute, Muang, Nakhon Ratchasima, 30000, Thailand

Danna Ye, Anawat Sangmalee, Rangsun Parnpai

Suranaree University of Technology, Institute of Agricultural Technology, Embryo Technology and Stem Cell Research Center, School of Biotechnology, Nakhon Ratchasiama, 30000 Thailand

Chanchao Lorthongpanich

Institute of Medical Biology, Mammalian Development Lab, A*STAR, 8A Biomedical Grove, Singapore 138648

Philip Heraud

Monash University, Monash Immunology and Stem Cell Laboratories, Clayton, Victoria, Melbourne, 3800 Australia

Monash University, Centre for Biospectroscopy, Clayton, Victoria, Melbourne, 3800 Australia

J. Biomed. Opt. 16(5), 057005 (May 23, 2011). doi:10.1117/1.3580253
History: Received August 27, 2010; Revised March 25, 2011; Accepted March 25, 2011; Published May 23, 2011; Online May 23, 2011
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Stem cell–based therapy for liver regeneration has been proposed to overcome the persistent shortage in the supply of suitable donor organs. A requirement for this to succeed is to find a rapid method to detect functional hepatocytes, differentiated from embryonic stem cells. We propose Fourier transform infrared (FTIR) microspectroscopy as a versatile method to identify the early and last stages of the differentiation process leading to the formation of hepatocytes. Using synchrotron-FTIR microspectroscopy, the means of identifying hepatocytes at the single-cell level is possible and explored. Principal component analysis and subsequent partial least-squares (PLS) discriminant analysis is applied to distinguish endoderm induction from hepatic progenitor cells and matured hepatocyte-like cells. The data are well modeled by PLS with endoderm induction, hepatic progenitor cells, and mature hepatocyte-like cells able to be discriminated with very high sensitivity and specificity. This method provides a practical tool to monitor endoderm induction and has the potential to be applied for quality control of cell differentiation leading to hepatocyte formation.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Kanjana Thumanu ; Waraporn Tanthanuch ; Danna Ye ; Anawat Sangmalee ; Chanchao Lorthongpanich, et al.
"Spectroscopic signature of mouse embryonic stem cell–derived hepatocytes using synchrotron Fourier transform infrared microspectroscopy", J. Biomed. Opt. 16(5), 057005 (May 23, 2011). ; http://dx.doi.org/10.1117/1.3580253


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