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Research Papers: Therapeutic

Image-guided genomic analysis of tissue response to laser-induced thermal stress

[+] Author Affiliations
Mark A. Mackanos

Stanford University School of Medicine, Department of Pediatrics, Clark Center E-150, 318 Campus Drive, Stanford, California 94305-5427

Stanford Infrared Optics and Photomedicine Center, Clark Center E-150, 318 Campus Drive, Stanford, California 94305-5427

Stanford University, Hansen Experimental Physics Laboratory, HEPL, 452 Lomita Mall, Stanford University, Stanford, California 94305-4085

Molecular Imaging Program at Stanford (MIPS) and BioX Program, Clark Center, East Wing First Floor, 318 Campus Drive, Stanford, California 94305-5427

Mike Helms

Stanford University School of Medicine, Department of Pediatrics, Clark Center E-150, 318 Campus Drive, Stanford, California 94305-5427

Molecular Imaging Program at Stanford (MIPS) and BioX Program, Clark Center, East Wing First Floor, 318 Campus Drive, Stanford, California 94305-5427

Cenix BioScience, Tatzberg 47, 01309 Dresden, Germany

Flora Kalish

Stanford University School of Medicine, Department of Pediatrics, Clark Center E-150, 318 Campus Drive, Stanford, California 94305-5427

Molecular Imaging Program at Stanford (MIPS) and BioX Program, Clark Center, East Wing First Floor, 318 Campus Drive, Stanford, California 94305-5427

Christopher H. Contag

Stanford University School of Medicine, Department of Pediatrics, Clark Center E-150, 318 Campus Drive, Stanford, California 94305-5427

Stanford Infrared Optics and Photomedicine Center, Clark Center E-150, 318 Campus Drive, Stanford, California 94305-5427

Stanford University, Hansen Experimental Physics Laboratory, HEPL, 452 Lomita Mall, Stanford University, Stanford, California 94305-4085

Molecular Imaging Program at Stanford (MIPS) and BioX Program, Clark Center, East Wing First Floor, 318 Campus Drive, Stanford, California 94305-5427

Stanford University School of Medicine, Department of Microbiology and Immunology, Fairchild Science Building, D300, 299 Campus Drive, Stanford, California 94305-5124

Stanford University School of Medicine, Department of Radiology, 300 Pasteur Drive, Stanford, California 94305-5105

J. Biomed. Opt. 16(5), 058001 (August 19, 2010March 07, 2011March 09, 2011May 12, 2011May 12, 2011). doi:10.1117/1.3573387
History: Received August 19, 2010; Revised March 07, 2011; Accepted March 09, 2011; Published May 12, 2011; Online May 12, 2011
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The cytoprotective response to thermal injury is characterized by transcriptional activation of “heat shock proteins” (hsp) and proinflammatory proteins. Expression of these proteins may predict cellular survival. Microarray analyses were performed to identify spatially distinct gene expression patterns responding to thermal injury. Laser injury zones were identified by expression of a transgene reporter comprised of the 70 kD hsp gene and the firefly luciferase coding sequence. Zones included the laser spot, the surrounding region where hsp70-luc expression was increased, and a region adjacent to the surrounding region. A total of 145 genes were up-regulated in the laser irradiated region, while 69 were up-regulated in the adjacent region. At 7 hours the chemokine Cxcl3 was the highest expressed gene in the laser spot (24 fold) and adjacent region (32 fold). Chemokines were the most common up-regulated genes identified. Microarray gene expression was successfully validated using qRT- polymerase chain reaction for selected genes of interest. The early response genes are likely involved in cytoprotection and initiation of the healing response. Their regulatory elements will benefit creating the next generation reporter mice and controlling expression of therapeutic proteins. The identified genes serve as drug development targets that may prevent acute tissue damage and accelerate healing.

Figures in this Article
© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Topics

Lasers ; Tissues

Citation

Mark A. Mackanos ; Mike Helms ; Flora Kalish and Christopher H. Contag
"Image-guided genomic analysis of tissue response to laser-induced thermal stress", J. Biomed. Opt. 16(5), 058001 (August 19, 2010March 07, 2011March 09, 2011May 12, 2011May 12, 2011). ; http://dx.doi.org/10.1117/1.3573387


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