Research Papers: Sensing

Photoacoustic spectroscopy of ovarian normal, benign, and malignant tissues: a pilot study

[+] Author Affiliations
Sudha D. Kamath

Manipal University, Manipal Life Sciences Centre, Biophysics Unit, Manipal, India

Manipal University, Manipal Institute of Technology, Department of Physics, Manipal, India

Satadru Ray

Manipal University, Kasturba Medical College, Department of Surgical Oncology, Manipal, India

Krishna K. Mahato

Manipal University, Manipal Life Sciences Centre, Biophysics Unit, Manipal, India

J. Biomed. Opt. 16(6), 067001 (June 01, 2011). doi:10.1117/1.3583573
History: Received June 25, 2010; Revised March 24, 2011; Accepted April 06, 2011; Published June 01, 2011; Online June 01, 2011
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Photoacoustic spectra of normal, benign, and malignant ovarian tissues are recorded using 325-nm pulsed laser excitation in vitro. A total of 102 (34 normal, 38 benign, and 30 malignant) spectra are obtained from 22 samples belonging to normal, benign, and malignant subjects. Applying multi-algorithm approach, comprised of methods such as, principal component analysis (PCA) based k-nearest neighbor (k-NN) analysis, artificial neural network (ANN) analysis, and support vector machine (SVM) analysis, classification of the data has been carried out. For PCA, first the calibration set is formed by pooling 45 spectra, 15 belonging to each of pathologically certified normal, benign, and malignant samples. PCA is then performed on the data matrix, comprised of the six spectral features extracted from each of 45 calibration samples, and three principal components (PCs) containing maximum diagnostic information are selected. The scores of the selected PCs are used to train the k-NN, ANN, and SVM classifiers. The ANN used is a classical multilayer feed forward network with back propagation algorithm for its training. For k-NN, the Euclidean distance based algorithm is used and for SVM, one-versus-rest multiclass kernel-radial basis function is used. The performance evaluation of the classification results are obtained by calculating statistical parameters like specificity and sensitivity. ANN and k-NN techniques showed identical performance with specificity and sensitivity values of 100 and 86.76%, whereas SVM had these values at 100 and 80.18%, respectively. In order to determine the relative diagnostic performance of the techniques, receiver operating characteristics analysis is also performed.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Sudha D. Kamath ; Satadru Ray and Krishna K. Mahato
"Photoacoustic spectroscopy of ovarian normal, benign, and malignant tissues: a pilot study", J. Biomed. Opt. 16(6), 067001 (June 01, 2011). ; http://dx.doi.org/10.1117/1.3583573


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