Research Papers: Sensing

Label-free and noninvasive optical detection of the distribution of nanometer-size mitochondria in single cells

[+] Author Affiliations
Xuantao Su

Shandong University, School of Control Science & Engineering, Department of Biomedical Engineering, Jinan, China 250061

University of Alberta, Department of Electrical and Computer Engineering, Edmonton, Alberta, T6G 2V4 Canada

Yuanyuan Qiu, Leah Marquez-Curtis

Canadian Blood Services, Edmonton, Alberta, T6G 2R8 Canada

Manisha Gupta, Clarence E. Capjack, Ying Y. Tsui

University of Alberta, Department of Electrical and Computer Engineering, Edmonton, Alberta, T6G 2V4 Canada

Wojciech Rozmus

University of Alberta, Department of Physics, Edmonton, Alberta, T6G 2G7 Canada

Anna Janowska-Wieczorek

Canadian Blood Services, Edmonton, Alberta, T6G 2R8 Canada

University of Alberta, Department of Medicine, Edmonton, Alberta, T6G 2V2 Canada

J. Biomed. Opt. 16(6), 067003 (June 01, 2011). doi:10.1117/1.3583577
History: Received November 16, 2010; Revised March 28, 2011; Accepted April 07, 2011; Published June 01, 2011; Online June 01, 2011
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A microfluidic flow cytometric technique capable of obtaining information on nanometer-sized organelles in single cells in a label-free, noninvasive optical manner was developed. Experimental two-dimensional (2D) light scattering patterns from malignant lymphoid cells (Jurkat cell line) and normal hematopoietic stem cells (cord blood CD34+ cells) were compared with those obtained from finite-difference time-domain simulations. In the simulations, we assumed that the mitochondria were randomly distributed throughout a Jurkat cell, and aggregated in a CD34+ cell. Comparison of the experimental and simulated light scattering patterns led us to conclude that distinction from these two types of cells may be due to different mitochondrial distributions. This observation was confirmed by conventional confocal fluorescence microscopy. A method for potential cell discrimination was developed based on analysis of the 2D light scattering patterns. Potential clinical applications using mitochondria as intrinsic biological markers in single cells were discussed in terms of normal cells (CD34+ cell and lymphocytes) versus malignant cells (THP-1 and Jurkat cell lines).

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Xuantao Su ; Yuanyuan Qiu ; Leah Marquez-Curtis ; Manisha Gupta ; Clarence E. Capjack, et al.
"Label-free and noninvasive optical detection of the distribution of nanometer-size mitochondria in single cells", J. Biomed. Opt. 16(6), 067003 (June 01, 2011). ; http://dx.doi.org/10.1117/1.3583577


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