0
Research Papers: Therapeutic

Reduced nicotinamide adenine dinucleotide fluorescence lifetime detected poly(adenosine-5′-diphosphate-ribose) polymerase-1-mediated cell death and therapeutic effect of pyruvate

[+] Author Affiliations
Han-Wen Guo, Hsing-Wen Wang

National Yang-Ming University, Institute of Biophotonics, Taipei 112, Taiwan

Yau-Huei Wei

National Yang-Ming University, Institute of Biochemistry and Molecular Biology, Taipei 112, Taiwan

Mackay Medical College, Department of Medicine, SanJhih, Taipei County 252, Taiwan

J. Biomed. Opt. 16(6), 068001 (June 09, 2011). doi:10.1117/1.3590204
History: Received January 17, 2011; Revised April 08, 2011; Accepted April 11, 2011; Published June 09, 2011; Online June 09, 2011
Text Size: A A A

Noninvasive detection of cell death has the potential for definitive diagnosis and monitoring treatment outcomes in real time. Reduced nicotinamide adenine dinucleotide (NADH) fluorescence intensity has long been used as a noninvasive optical probe of metabolic states. NADH fluorescence lifetime has recently been studied for its potential as an alternative optical probe of cellular metabolic states and cell death. In this study, we investigated the potential using NADH fluorescence intensity and/or lifetime to detect poly(adenosine-5′-diphosphate-ribose) polymerase-1 (PARP-1)-mediated cell death in HeLa cells. We also examined if NADH signals respond to treatment by pyruvate. The mechanism of PARP-1-mediated cell death has been well studied that extensive PARP-1 activation leads to cytosolic nicotinamide adenine dinucleotide depletion resulting in glycolytic inhibition, mitochondrial failure, and death. Pyruvate could restore electron transport chain to prevent energy failure and death. Our results show that NADH fluorescence lifetime, not intensity, responded to PARP-1-mediated cell death and the rescue effect of pyruvate. This lifetime change of NADH fluorescence happened before the collapse of mitochondrial membrane potential and mitochondrial uncoupling. Together with our previous findings in staurosporine-induced cell death, we suggest that NADH fluorescence lifetime increase during cell death is mainly due to increased protein-protein interactions but not the intracellular NADH content.

Figures in this Article
© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Han-Wen Guo ; Yau-Huei Wei and Hsing-Wen Wang
"Reduced nicotinamide adenine dinucleotide fluorescence lifetime detected poly(adenosine-5′-diphosphate-ribose) polymerase-1-mediated cell death and therapeutic effect of pyruvate", J. Biomed. Opt. 16(6), 068001 (June 09, 2011). ; http://dx.doi.org/10.1117/1.3590204


Access This Article
Sign In to Access Full Content
Please Wait... Processing your request... Please Wait.
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
 
Your Session has timed out. Please sign back in to continue.
Sign In to Access Full Content

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement

Buy this article ($18 for members, $25 for non-members).
Sign In