Research Papers: General

Backward emission angle of microscopic second-harmonic generation from crystallized type I collagen fiber

[+] Author Affiliations
Long Tian, Huajiang Wei, Ying Jin, Hanping Liu, Zhouyi Guo

South China Normal University, MOE Key Laboratory of Laser Life Science, Tianhe Shipai, Guangzhou, Guangzhou 510631 China

Xiaoyuan Deng

South China Normal University, MOE Key Laboratory of Laser Life Science, Tianhe Shipai, Guangzhou, Guangzhou 510631 China

South China Normal University, Research Resources Center, Guangzhou Higher Education Mega Center, Guangzhou, Guangzhou 510006 China

J. Biomed. Opt. 16(7), 075001 (July 01, 2011). doi:10.1117/1.3596174
History: Received February 15, 2011; Revised April 25, 2011; Accepted May 11, 2011; Published July 01, 2011; Online July 01, 2011
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A theoretical model that deals with SHG from crystallized type I collagen fiber formed by a bundle of fibrils is established. By introducing a density distribution function of dipoles within fibrils assembly into the dipole theory and combining with structural order (m,l) parameters revealed by quasi-phase-matching (QPM) theory, our established theoretical model comprehensively characterizes both biophysical features of collagen dipoles and the crystalline characteristics of collagen fiber. This new model quantitatively reveals the 3-D distribution of second-harmonic generation (SHG) emission angle (θ,φ) in accordance with the emission power. Results show that fibrils diameter d1 and structural order m, which describes the structural characteristics of collagen fiber along the incident light propagation direction has significant influence on backward/forward SHG emission. The decrease of fibrils diameter d1 induces an increase of the peak SHG emission angle θmax. As d1 decreases to a threshold value, in our case it is around d1 = 150 nm when (m,l) = (1,0), θmax > 90 deg, indicating that backward SHG emission appears. The SHG may have two symmetrical emission distribution lobes or may have only one or two unsymmetrical emission lobes with unequal emission power, depending on the functional area of (m,l) on d1.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Long Tian ; Huajiang Wei ; Ying Jin ; Hanping Liu ; Zhouyi Guo, et al.
"Backward emission angle of microscopic second-harmonic generation from crystallized type I collagen fiber", J. Biomed. Opt. 16(7), 075001 (July 01, 2011). ; http://dx.doi.org/10.1117/1.3596174


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