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Research Papers: General

Viability of fibroblasts cultured under nutritional stress irradiated with red laser, infrared laser, and red light-emitting diode

[+] Author Affiliations
Luiz Evaristo Ricci Volpato

University of Cuiabá, Cuiabá School of Dentistry, Rua Estevão de Mendonça, 317 apto 501, Goiabeiras, Cuiabá, MT, 78065-480 Brazil

Rodrigo Cardoso de Oliveira, Maria A. A. M. Machado

University of São Paulo, Bauru School of Dentistry, Al. Dr. Octávio Pinheiro Brisolla, 9-75 Bauru, SP, 17012-901 Brazil

Mariano Martinez Espinosa

Federal University of Mato Grosso, Department of Statistics, Avenida Fernando Corrêa, s/nº Coxipó, Cuiabá, MT, 78060-900 Brazil

Vanderley Salvador Bagnato

University of São Paulo, Physics Institute of São Carlos, Av. Trabalhador São-Carlense, 400 São Carlos, SP, 13566-590 Brazil

J. Biomed. Opt. 16(7), 075004 (March 23, 2011April 18, 2011June 02, 2011July 08, 2011July 08, 2011). doi:10.1117/1.3602850
History: Received March 23, 2011; Revised April 18, 2011; Accepted June 02, 2011; Published July 08, 2011; Online July 08, 2011
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Phototherapy is noninvasive, painless and has no known side effect. However, for its incorporation into clinical practice, more well-designed studies are necessary to define optimal parameters for its application. The viability of fibroblasts cultured under nutritional stress irradiated with either a red laser, an infrared laser, or a red light-emitting diode (LED) was analyzed. Irradiation parameters were: red laser (660 nm, 40 mW, 1 W/cm2), infrared laser (780 nm, 40 mW, 1 W/cm2), and red LED (637 ± 15 nm, 40 mW, 1 W/cm2). All applications were punctual and performed with a spot with 0.4 mm2 of diameter for 4 or 8 s. The Kruskal–Wallis test and analysis of variance of the general linear model (p ≤ 0.05) were used for statistical analysis. After 72 h, phototherapy with low-intensity laser and LED showed no toxicity at the cellular level. It even stimulated methylthiazol tetrazolium assay (MTT) conversion and neutral red uptake of fibroblasts cultured under nutritional stress, especially in the group irradiated with infrared laser (p = 0.004 for MTT conversion and p < 0.001 for neutral red uptake). Considering the parameters and protocol of phototherapy used, it can be concluded that phototherapy stimulated the viability of fibroblasts cultured under nutritional deficit resembling those found in traumatized tissue in which cell viability is reduced.

Figures in this Article
© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Luiz Evaristo Ricci Volpato ; Rodrigo Cardoso de Oliveira ; Mariano Martinez Espinosa ; Vanderley Salvador Bagnato and Maria A. A. M. Machado
"Viability of fibroblasts cultured under nutritional stress irradiated with red laser, infrared laser, and red light-emitting diode", J. Biomed. Opt. 16(7), 075004 (March 23, 2011April 18, 2011June 02, 2011July 08, 2011July 08, 2011). ; http://dx.doi.org/10.1117/1.3602850


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