Research Papers: Imaging

Photoacoustic microscopy of microvascular responses to cortical electrical stimulation

[+] Author Affiliations
Vassiliy Tsytsarev

Washington University in St. Louis, Department of Biomedical Engineering, One Brookings Drive, St. Louis, Missouri 63130

George Mason University, Krasnow Institute for Advanced Study, Molecular Neuroscience Department, 4400 University Drive, Fairfax, Virginia 22030

Song Hu, Junjie Yao, Konstantin Maslov, Dennis L. Barbour, Lihong V. Wang

Washington University in St. Louis, Department of Biomedical Engineering, One Brookings Drive, St. Louis, Missouri 63130

J. Biomed. Opt. 16(7), 076002 (July 01, 2011). doi:10.1117/1.3594785
History: Received October 21, 2010; Revised April 04, 2011; Accepted May 05, 2011; Published July 01, 2011; Online July 01, 2011
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Advances in the functional imaging of cortical hemodynamics have greatly facilitated the understanding of neurovascular coupling. In this study, label-free optical-resolution photoacoustic microscopy (OR-PAM) was used to monitor microvascular responses to direct electrical stimulations of the mouse somatosensory cortex through a cranial opening. The responses appeared in two forms: vasoconstriction and vasodilatation. The transition between these two forms of response was observed in single vessels by varying the stimulation intensity. Marked correlation was found between the current-dependent responses of two daughter vessels bifurcating from the same parent vessel. Statistical analysis of twenty-seven vessels from three different animals further characterized the spatial-temporal features and the current dependence of the microvascular response. Our results demonstrate that OR-PAM is a valuable tool to study neurovascular coupling at the microscopic level.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Vassiliy Tsytsarev ; Song Hu ; Junjie Yao ; Konstantin Maslov ; Dennis L. Barbour, et al.
"Photoacoustic microscopy of microvascular responses to cortical electrical stimulation", J. Biomed. Opt. 16(7), 076002 (July 01, 2011). ; http://dx.doi.org/10.1117/1.3594785


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