Research Papers: Imaging

Label-free oxygen-metabolic photoacoustic microscopy in vivo

[+] Author Affiliations
Junjie Yao, Konstantin I. Maslov, Yu Zhang, Younan Xia, Lihong V. Wang

Washington University in St. Louis, Department of Biomedical Engineering, One Brookings Drive, St. Louis, Missouri 63130

J. Biomed. Opt. 16(7), 076003 (July 01, 2011). doi:10.1117/1.3594786
History: Received February 17, 2011; Revised April 12, 2011; Accepted May 05, 2011; Published July 01, 2011; Online July 01, 2011
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Almost all diseases, especially cancer and diabetes, manifest abnormal oxygen metabolism. Accurately measuring the metabolic rate of oxygen (MRO2) can be helpful for fundamental pathophysiological studies, and even early diagnosis and treatment of disease. Current techniques either lack high resolution or rely on exogenous contrast. Here, we propose label-free metabolic photoacoustic microscopy (mPAM) with small vessel resolution to noninvasively quantify MRO2in vivo in absolute units. mPAM is the unique modality for simultaneously imaging all five anatomical, chemical, and fluid-dynamic parameters required for such quantification: tissue volume, vessel cross-section, concentration of hemoglobin, oxygen saturation of hemoglobin, and blood flow speed. Hyperthermia, cryotherapy, melanoma, and glioblastoma were longitudinally imaged in vivo. Counterintuitively, increased MRO2 does not necessarily cause hypoxia or increase oxygen extraction. In fact, early-stage cancer was found to be hyperoxic despite hypermetabolism.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Junjie Yao ; Konstantin I. Maslov ; Yu Zhang ; Younan Xia and Lihong V. Wang
"Label-free oxygen-metabolic photoacoustic microscopy in vivo", J. Biomed. Opt. 16(7), 076003 (July 01, 2011). ; http://dx.doi.org/10.1117/1.3594786


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