Research Papers: Sensing

Pterin detection using surface-enhanced Raman spectroscopy incorporating a straightforward silver colloid–based synthesis technique

[+] Author Affiliations
Ciarán A. Smyth, Sam Mehigan, Yury P. Rakovich, Eithne M. McCabe

Trinity College Dublin, Semiconductor Photonics Group, School of Physics, Dublin 2, Ireland

Steven E. J. Bell

Queen's University Belfast, School of Chemistry and Chemical Engineering, Belfast BT95AG United Kingdom

J. Biomed. Opt. 16(7), 077007 (July 08, 2011). doi:10.1117/1.3600658
History: Received March 09, 2011; Revised May 12, 2011; Accepted May 23, 2011; Published July 08, 2011; Online July 08, 2011
Text Size: A A A

Optical techniques toward the realization of sensitive and selective biosensing platforms have received considerable attention in recent times. Techniques based on interferometry, surface plasmon resonance, and waveguides have all proved popular, while spectroscopy in particular offers much potential. Raman spectroscopy is an information-rich technique in which the vibrational frequencies reveal much about the structure of a compound, but it is a weak process and offers poor sensitivity. In response to this problem, surface-enhanced Raman scattering (SERS) has received much attention, due to significant increases in sensitivity instigated by bringing the sample into contact with an enhancing substrate. Here we discuss a facile and rapid technique for the detection of pterins using SERS-active colloidal silver suspensions. Pterins are a family of biological compounds that are employed in nature in color pigmentation and as facilitators in metabolic pathways. In this work, small volumes of xanthopterin, isoxanthopterin, and 7,8-dihydrobiopterin have been examined while adsorbed to silver colloids. Limits of detection have been examined for both xanthopterin and isoxanthopterin using a 10-s exposure to a 12 mW 532 nm laser, which, while showing a trade-off between scan time and signal intensity, still provides the opportunity for the investigation of simultaneous detection of both pterins in solution.

Figures in this Article
© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Ciarán A. Smyth ; Sam Mehigan ; Yury P. Rakovich ; Steven E. J. Bell and Eithne M. McCabe
"Pterin detection using surface-enhanced Raman spectroscopy incorporating a straightforward silver colloid–based synthesis technique", J. Biomed. Opt. 16(7), 077007 (July 08, 2011). ; http://dx.doi.org/10.1117/1.3600658


Tables

Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.