Research Papers: Imaging

Functional laser speckle imaging of cerebral blood flow under hypothermia

[+] Author Affiliations
Minheng Li, Peng Miao, Yisheng Zhu

Shanghai Jiao Tong University, School of Biomedical Engineering, Shanghai 200240, China

Shanbao Tong

Shanghai Jiao Tong University, School of Biomedical Engineering, Shanghai 200240, China

Shanghai Jiao Tong University, Med-X Research Institute, Shanghai 200030, China

J. Biomed. Opt. 16(8), 086011 (August 01, 2011). doi:10.1117/1.3610995
History: Received December 15, 2010; Revised June 10, 2011; Accepted June 22, 2011; Published August 01, 2011; Online August 01, 2011
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* Address all correspondence to: Shanbao Tong, Shanghai Jiao Tong University, School of Biomedical Engineering, 800 Dongchuan Road, Shanghai 200240, China; Tel: +86-21-34205138; Fax: +86-21-34204717; E-mail: stong@sjtu.edu.cn.

Hypothermia can unintentionally occur in daily life, e.g., in cardiovascular surgery or applied as therapeutics in the neurosciences critical care unit. So far, the temperature-induced spatiotemporal responses of the neural function have not been fully understood. In this study, we investigated the functional change in cerebral blood flow (CBF), accompanied with neuronal activation, by laser speckle imaging (LSI) during hypothermia. Laser speckle images from Sprague-Dawley rats (n = 8, male) were acquired under normothermia (37°C) and moderate hypothermia (32°C). For each animal, 10 trials of electrical hindpaw stimulation were delivered under both temperatures. Using registered laser speckle contrast analysis and temporal clustering analysis (TCA), we found a delayed response peak and a prolonged response window under hypothermia. Hypothermia also decreased the activation area and the amplitude of the peak CBF. The combination of LSI and TCA is a high-resolution functional imaging method to investigate the spatiotemporal neurovascular coupling in both normal and pathological brain functions.

Figures in this Article

Hypothermia occurs in many situations in life, e.g., exposure to cold environmental temperature, drowning, anesthesia, and cardiac surgery. Although a series of physiological changes following hypothermia is considered life threatening, the cooling procedure has been applied in the neuroscience critical care unit as neuroprotection since the early 1950s.12 Hypothermia is one of the most studied and promising neuroprotective approaches recommended by the Stroke Therapy Academic Industry Roundtable.34

Although the precise mechanisms of hypothermic neuroprotection are still unclear, the preservation of brain adenosine triphosphate (ATP) supply following the decrease in metabolism plays an important role.5 Several metabolic biochemical processes are also disturbed by hypothermia: the release of amino acids6 and the formation of excitatory neurotransmitters, hydroxyl radicals,7 and nitric oxide.8 These processes are linked to the reduction of neuronal activity9 and the mediation of coupling brain activity to cerebral blood flow (CBF).10

Nevertheless, the effect of hypothermia on the spatiotemporal neurovascular response to functional stimulation has not been investigated well. Investigating the coupling between vascular responses and functional activations under hypothermia will help 1. further understand the neurovascular coupling involved in the thermoregulation of the brain and 2. monitor the neuronal function in clinical therapeutic hypothermia.

Laser speckle imaging (LSI)1112 of neurovascular coupling has gained considerable importance in both clinical13 and laboratory settings14 because of its advantage in spatiotemporal resolution over other methods, including conventional laser Doppler flowmetry (LDF),15 and new techniques such as functional magnetic resonance imaging (fMRI) and positron emission tomography. In this study, we used LSI to investigate neurovascular coupling after hindpaw electrical stimulation under hypothermia. Somatosensory stimulation was delivered with current pulses (i.e., 5 Hz, 2.5 mA, and 0.3 ms) for 4 s under normothermia (37°C) or hypothermia (32°C).

Using registered laser speckle contrast analysis (rLASCA)16 and temporal clustering analysis (TCA), we obtained the spatiotemporal profile of the functional activation altered by hypothermia. LSI bridges the gap between neuronal activity and vascular studies with high spatiotemporal resolution, and facilitates the study of functional organization of cortical microcirculation and the adaptive regulation of CBF triggered by physiologic or pathologic stimulus.

Animal Preparation

Eight male Sprague-Dawley rats (270 ± 20g, Slac Laboratory Animal, Shanghai, China) were anesthetized with sodium pentobarbital (60 mg/kg, IP). The animals were constrained in a stereotaxic frame (Benchmark DeluxeTM, MyNeurolab.com, St. Louis, Missouri) during the experiment. Rectal temperature was monitored and maintained at 37.0 ± 0.2°C with a heating pad and DC control module (FHC Inc., Bowdoinham, Maine). Blood pressure (BP) and heart rate (HR) of each rat were continuously monitored by the tail-cuff method using an automatic sphygmomanometer (Softron BP-98A system, Softron Co., Tokyo, Japan). A midline incision was made on the scalp, and tissues were cleaned to expose the surface of the skull with a scalpel. An ∼8×5 mm2 cranial window over the right somatosensory cortex was thinned to translucency by a high-speed dental drill (SDE-H37L, Marathon, Korea) with a 1.6 mm burr (Dentsply, Switzerland). Saline was used for cooling during the smoothing. Glycerol was used to reduce the specular reflections and improve the optical clearing.17

Electrical Hindpaw Stimulation

Hindpaw stimulation was carried out by a programmed stimulator (YC-2, Cheng Yi, China) using two needle electrodes subcutaneously inserted. Each stimulation block involved 10 sequential trials. Each trial consisted of baseline (4 s), stimulation [4 s, rectangular constant current pulses (2.5 mA, 0.3 ms, 5 Hz)], and post-stimulus period (12 s). The minimum of the intertrial interval was 60 s.

Hypothermia Procedure

The experimental protocols, approved by the animal care and usage committee of Shanghai Jiao Tong University, include normothermia, cooling, and moderate hypothermia sessions. After the surgical preparation, temperature was kept stable at 37 ± 0.2°C for 10 min, and stimulations to the left hindpaw were performed every minute. Hypothermia was induced by swabbing the rat's skin with an alcohol pad under an electric fan, as done in previous literature.1819 When the temperature was close to 32.5°C, the animal was allowed to cool down passively until the target temperature was reached. The entire cooling procedure took about 40 min. The temperature was then maintained at 32 ± 0.5°C using a feedback-controlled heating pad. During hypothermia, the hindpaw stimulations were delivered in the same way as in the normothermic stage. After the experiments, the animals were euthanized by an overdose of pentobarbital.

Imaging Procedure

The laser speckle images (696×512 pixels) were acquired at 23 fps (exposure time T = 5 ms) with a 12-bit CCD camera (Pixelfly QE, Cooke, USA) mounted on a trinocular stereo microscope (XYH-05, Yongheng, Shanghai, China) over the thinned skull. A laser diode (635 ± 2 nm, 10 mW, Model KL5650, Forward Co., Ltd., Shanghai, China) was used to illuminate the region of interest (ROI). In each trial, we recorded 460 consecutive frames (i.e., 20 s) of speckle images. The stimulation was programmed to start at the fifth second.

Data Processing
Registering the laser speckle imaging data

To suppress noises due to respiration, heart beating, or both, we first aligned the raw LSI images by the rLASCA method proposed by our group.16 After the raw speckle images were registered with a 3×3 convolution kernel, normalized correlation metric, and cubic B-spline interpolator, we constructed the contrast values for the CBF analysis based on the registered images.

Measurement of blood speed

The principle of LSI has been previously described in literature.11,2021 Briefly, when a highly coherent laser source illuminates a rough surface or a medium that contains moving particles, e.g., blood cells, the interference of the reflected or scattered light results in a granular pattern known as speckle. The most common measure for the speckle pattern related to the speed of the scattering particles is the laser speckle contrast analysis (LASCA),11 which is defined as the ratio of the standard deviation (SD) to the mean intensity in a small region of the image.22 Each raw image was scanned with a sliding window (5×5 pixels) to calculate the speckle contrast, ensuring the sufficiently accurate estimation of speckle contrast with a limited cost of spatial resolution. In the case of blood flow, speckle contrast Ks is theoretically linked to the speed by Display Formula

1Ks=σSI=β1/2τcT+τc22T2exp2Tτc11/2,
where T is the exposure time of the CCD and the autocorrelation time τc is assumed to be inversely and linearly proportional to the mean speed of the blood flow.11 β accounts for the loss of correlation, which is related to the ratio of the detector size to the speckle size and polarization.23 In the single dynamic scattering regime, Ref. 24 confirms that Eq. 1 is correct if the moving particles undergo Brownian motion. When photons scatter no more than once from the moving particles in the single dynamic scattering regime, the argument of the exponent in Eq. 1 should be squared to represent blood speed.24 Furthermore, the relation between τc and the speed of the scattering particles still depends on the speed distribution of the particles sampled by the detected light.2526 Although the values of Ks2 are in the range of [0, 1] in theory, they are practically limited to [0, 0.1]. Moreover, Ks2 degrades into a linear correlation with τc when the contrast is less than 0.1, according to Eq. 1. Thus, we use its reciprocal (Cs=1/Ks2) hereafter to calculate the relative changes in the CBF.

All images were filtered with a Gaussian matrix (half width = 5 pixels) to remove the spatial noises. Contrast values for every 11 frames of consecutive speckle images (∼0.5 s) were averaged to obtain one blood flow image (Cs). To increase the signal-to-noise ratio further, we used the average of Cs over 10 trials to analyze the CBF change. All image processing was carried out in Matlab (Mathworks, Massachusetts).

Original temporal clustering analysis method

To analyze the spatiotemporal profile of brain activations, previous studies were usually based on a priori assumptions of the temporal activation patterns. Liu et al. 27 and Gao et al. 28 proposed TCA to detect the time windows of the maximal brain responses in fMRI. In this study, TCA was used to identify the pattern of brain activation regardless of anatomical variances because of the great spatiotemporal heterogeneity in the cortical vasculature.29 Mathematically, TCA converts a high-dimensional space into a one-dimensional space to obtain the number of voxels that reach the maximum of activation at each time point.27 In the current study, we implemented the original TCA (OTCA) method to analyze the LASCA images and detect the stimulus-induced temporal CBF changes under different temperatures. The CBF response was computed within a 2.1×2.1 mm2 (200×200 pixels) ROI over the primary somatosensory hindpaw cortex.

The theory of OTCA is detailed in Ref. 27 and 28. Briefly, the dataset Cs (t frames with m×n pixels each) can be represented as a two-dimensional matrix C as follows: Display Formula

2C=Cs1,1Cs1,2Cs1,3Cs1,tCs2,1Cs2,2Cs2,3Cs2,tCs3.1Cs3,2Cs3,3Cs3,tCsm×n,1Csm×n,2Csm×n,3Csm×n,t,
with each column (Cs1,j,Cs2,j,...,Csm×n,j) representing the intensities of all pixels in the jth frame. The matrix C is further normalized by the intensities in each pixel for the baseline (i.e., Ci,0) Display Formula
3Vi,j=|Ci,jCi,0|Ci,0(1im×n,1jt).
To find the maximum change at each time point, a temporary matrix W is created to determine whether the ith pixel reaches the maximum at the jth time point Display Formula
4Wi,j=1,ifVi,j=maxVi,1,Vi,2,Vi,3,...,Vi,t0,otherwise.
The pixels with maximum changes at time j {(i,j) | Wi,j = 1} are called temporal cluster at that time point. Thus, the summation along each column in W represents the size of the temporal cluster at the corresponding time point Display Formula
5N(t)=(N1,N2,N3,...,Nt),whereNj=i=1m×nWi,j.
N(t) is used to estimate the response peak and duration of the CBF by Gaussian regression.

Statistical Analysis

Statistical analysis was carried out with SPSS 15.0. Paired t-tests were used to analyze the significance of the differences in both response peak and duration between normothermic and hypothermic stages. The differences in the maximum activation area and peak CBF changes between normothermic and hypothermic phases were also analyzed by the same test. p < 0.05 implies a statistically significant difference. The tables and figures show the mean with SD.

The vital physiological parameters used in the experiments are listed in Table 1. Both HR and BP significantly dropped under hypothermia.

Table Grahic Jump Location
HR and BP under normothermia and hypothermia. (Data are presented as mean ± SD; *: paired t-test with hypothermia, P < 0.05.)
Laser Speckle Contrast Imaging of Cerebral Blood Flow under Functional Stimulation

Figure 1 illustrates the spatiotemporal changes in CBF averaged over 10 trials with 4 s hindpaw stimulation for one animal under normothermia (37°C). The images are shown at 0.5 s intervals, indicating an increase in the CBF approximately 1 s after the stimulus onset. To avoid the influence from small noises-derived fluctuations, only the CBF changes higher than 3% were overlaid over the speckle contrast images to show the spatial relationship between CBF change and cortical vascular structure. A prominent increase in CBF was detected around the 3 s post-stimulus onset, lasting for ∼3 s.

Graphic Jump LocationF1 :

Relative changes in CBF response to 4 s hindpaw stimulation in a typical animal under normothermia (37°C). The CBF changes higher than 3% are overlaid over the vasculature derived by speckle contrast images. Each image represents a 500 ms interval. A, anterior; P, posterior; L, lateral.

Figure 2 exhibits the spatiotemporal evolution of the CBF response to the same stimulation under hypothermia (32°C) for the same animal in Fig. 1. There was a delay in the response peak under hypothermia compared with normothermia. The CBF response peak occurred at nearly 4.5 s post-stimulus onset and was followed by a relatively more delayed and longer (∼5 s after the stimulation) recovery under hypothermia. Moreover, a smaller activated area and a lower CBF change under hypothermia were observed compared with those under normothermia.

Graphic Jump LocationF2 :

Relative changes in CBF response to 4 s hindpaw stimulation in a typical animal under hypothermia (32°C). The CBF changes higher than 3% are overlaid over the vasculature derived by speckle contrast images. Each image represents a 500 ms interval. A, anterior; P, posterior; L, lateral.

Time Courses of the Functional Activation under Different Temperatures

Figure 3 demonstrates the temporal profile of the functional activation under normothermia (37°C) and hypothermia (32°C) by the TCA method. To suppress the random noise caused by the trial and subject variations, the temporal clusters were pooled from 80 trials for eight rats.

Graphic Jump LocationF3 :

Spatiotemporal CBF changes response to the hindpaw stimulation under normothermia (37°C) and hypothermia (32°C). (a) Peak relative CBF change response to the hindpaw stimulation occurring 3 s after the stimulus onset under normothermia. (b) Peak relative CBF change response to the hindpaw stimulation occurring 5 s after the stimulus onset under hypothermia. The white boxes in (a) and (b) denote the ROI in measuring the spatial properties of the brain activation. (c) Temporal cluster of CBF response to the hindpaw stimulation. The TCA results are fitted with the Gaussian function (dotted line) used to detect the activation time window. For normothermia (37°C) (the red part), a time window (1.5 to 4.3 s) centered at tmax (2.9 s) is determined by the Gaussian fitting (r = 0.9511). For hypothermia (32°C) (the blue part), a time window (2.5 to 7.9 s) centered at tmax (5.2 s) is estimated by the Gaussian fitting (r = 0.9269). A, anterior; P, posterior; L, lateral.

To detect the activation peaks, the TCA results [Fig. 3] were fitted with the Gaussian function [i.e., N(t) in Eq. 5] Display Formula

6N(t)=A2πσ2e(ttmax)22σ2+B,
where σ is the standard deviation, and tmax is the time with the maximal CBF change or the response peak. Accordingly, we defined the full width at half maximum (FWHM) of N(t) as the response duration (Rd) centered at tmaxDisplay Formula
7Rd=FWHM=22ln2σ2.3548σ.
Figure 3 shows a longer response duration (2.5 to 7.9 s versus 1.5 to 4.3 s) and delayed response peak (5.2 s versus 2.9 s) under hypothermia (32°C) compared with normothermia (37°C). The correlation coefficients (r) of the Gaussian fitting were 0.9511 (37°C) and 0.9269 (32°C). It should be noted that three of the eight rats’ CBF responses to the stimulus underwent less Gaussian distribution than the remaining five, possibly implying a more complicated mathematical model under hypothermia, which needs more experimental data to fit.

Table 2 lists the statistical results of the response peak and duration under different temperatures averaged across eight animals based on 10 trials in each subject. The time to response peak was around 2.6 ± 0.4 s post-stimulus onset at 37°C, which was significantly delayed to 5.2 ± 1.2 s at 32°C (P < 0.05) under hypothermia. Furthermore, the response duration under normothermia (2.6 ± 0.6 s) was significantly prolonged under hypothermia (5.3 ± 2.3 s) (P < 0.05).

Table Grahic Jump Location
Spatial and temporal responses to 4 s hindpaw stimulation under normothermia and hypothermia (n = 8) in the aspect of relative CBF change. The time scale starts from the onset of a stimulus. (Data are presented as mean ± SD; *: paired t-test with hypothermia, P < 0.05.)

To show how much the CBF is correlated with the temperature, we present the scatter plots of the response peak [tmax, Fig. 4] and response duration [Rd, Fig. 4] under hypothermia versus normothermia for each animal. Linear regression analysis was performed to determine the correlation. The regression line in each plot was calculated according to y = ax, with y being the response values at hypothermia and x being the values at normothermia. Close correlations were observed between the responses (both tmax and Rd) under hypothermia and those under normothermia according to the correlation coefficients (tmax: r = 0.71; Rd: r = 0.82) with very similar slopes (tmax: s = 1.9271; Rd: s = 2.0955).

Graphic Jump LocationF4 :

Scatter plots showing the correlation of the (a) response peak (tmax) and (b) response duration (Rd) between normothermic and hypothermic stages. The best-fit line and correlation coefficient (R2) values represent the results of the linear regression analysis.

Effects of Temperature on Spatial Activation

The OTCA method does not directly provide the spatial details of the cortical response. To investigate the spatial properties of the brain activation under hypothermia, a ROI (150×150 pixels) window, which covered more than 60% of the pixels with CBF change above the half maximum of the response peak by OTCA, was defined. The activation area, i.e., pixels with CBF above the half maximum within the ROI,30 was used to quantify the CBF response.

Figure 3 illustrates the CBF change at tmax under normothermia [Fig. 3] and hypothermia [Fig. 3] for one animal. Table 2 shows the statistical results from eight animals, i.e., the activated area at tmax under normothermia is significantly larger than that under hypothermia (P < 0.05). There is also a statistically significant decrease in the peak relative CBF change (

ΔCBFtmax
) from normothermia to hypothermia (P < 0.05).

Figure 5 shows the relationship between the

ΔCBFtmax
under normothermia and that under hypothermia. An approximately linear correlation is observed for ΔCBFtmax between two temperatures (r = 0.56).

Graphic Jump LocationF5 :

Scatter plots showing the correlation of the peak relative CBF change (

ΔCBFtmax) between normothermic and hypothermic stages. The best-fit line and correlation coefficient (R2) values represent the results of the linear regression analysis.

Taking advantage of the high spatial resolution of LSI, we were able to find the different blood flow changes in a rat's primary somatosensory cortex through hindpaw stimulation in different vessels from six rats with distinguishable vasculature (Fig. 6). The CBF changes in the venules (ROI C in Fig. 6) were less prominent than those in the capillary bed and arteriolar vessels (ROI B in Fig. 6), in accordance with the different hemoglobin dynamics in the arterioles, capillaries, and venules.31 The arterioles and venules were manually segmented by the anatomical features.

Graphic Jump LocationF6 :

Blood flow change in rat's primary somatosensory cortex under hindpaw stimulation. Three ROI are selected for analyzing the CBF changes in different vessels; i.e., (a) whole activated area (ROI A) with two subregions for the capillary bed and arteriole (ROI B) and venule (ROI C). The CBF change in each rat is normalized by the maximum value of ROI B. The error bars represent the standard deviation. (b) Relative CBF changes in ROI. A, anterior; P, posterior; L, lateral.

Temporal Characteristics of Functional Activation

Under hypothermia, a significant delay of response was observed (P < 0.05), and the CBF recovered to the normal level (P < 0.05) more slowly. Under normothermia, CBF started to increase at ∼1 s and reached the peak at 2.6 ± 0.4 s after the stimulation onset, consistent with the reported hemodynamic changes during both forepaw32 and hindpaw33 stimulations in rats by LDF and LSI, respectively. In modeling the stimuli-induced hemodynamic responses, the Gaussian function has been proven to be a more natural and flexible mathematical model than the Poisson or Gamma function.34 It fits well with the data from optical intrinsic imaging and fMRI, especially in characterizing the time delay between the hemodynamic response and neural activities.3435 The correlation coefficients of the Gaussian fitting of our TCA data were compared between hypothermia and normothermia (0.9511 versus 0.9269), implying that the hemodynamic response still maintains the same temporal statistic model under hypothermia. The slower neuronal response under hypothermia may be caused by the suppression of the conduction in the peripheral nerve and spinal pathways36 under low temperature. Thus, it increases the latencies and prolongs the durations of the excitatory postsynaptic potentials evoked by electrical stimulation.37 The molecular mechanism of such delayed and prolonged response under hypothermia is probably related to the decreased release of neurotransmitters such as glutamate.2

Spatial Characteristics of Functional Activation

Another interesting question is whether hypothermia could alter the activity-induced neurovascular or neurometabolic coupling. Our LSI results show that both the activation area and

ΔCBFtmax
, in response to the functional hindpaw stimulation, were significantly reduced under hypothermia (P < 0.05). However, previous results from Royl et al. 38 show only the magnitude, and not the temporal features (i.e., latency and duration), of the relative CBF change under hypothermia that could have been caused by the limited spatial resolution of LDF. Fixed ROI chosen by traditional LDF would bias the relative CBF change because of the heterogeneous vasculature. OTCA solves this problem by defining the ROI dependent on the response results, which, therefore, provides higher spatiotemporal resolution of the functional representation of the CBF change without prior assumptions or knowledge on the spatiotemporal patterns of cortical activations. Note that the temporal profile of our CBF change by LSI is more consistent with Royl's finding on the electrophysiological response to somatosensory stimulation during hypothermia, i.e., the decreased amplitude and delayed peak time of somatosensory evoked potential (SEP), than the CBF results by the LDF method.38 Combining the results with other electroencephalogram (EEG) findings, e.g., a decrease in EEG amplitude and a shift of EEG waves to lower frequencies under hypothermia conditions,39 we can conclude that the neurovascular coupling is preserved during moderate hypothermia to some extent.

Most biological processes governing neuronal activity have a temperature coefficient (Q10) between 2 and 3.37 Q10 is calculated as follows: Display Formula

8Q10=R2R110/t2t1,
where R is the rate of change, and t is the temperature in Celsius degrees or Kelvin. In the current study, Q10 of ΔCBFtmax from normothermia to hypothermia is 2.35, which is according to the data in Fig. 5. This Q10 value is similar to that reported for CMRO2 suppression when the brain temperature decreases from 37 to 32°C.40 This finding indicates that CBF and metabolism share the same temperature dependence, which supports the report that the coupling between CBF and metabolism is maintained during moderate hypothermia.41

Furthermore, the reduced activation area and relative CBF change under hypothermia may imply that a smaller number of neurons are involved in functional activation. Nevertheless, considering the prolonged response duration under hypothermia, the total energy demand under hypothermia may not change compared with that under normothermia.

The spatial pattern of the vascular hemodynamic response is closely related to the neuronal function. So far, capillary responses to somatosensory stimulus have not been fully understood. Taking advantage of the high spatial resolution of LSI, we were able to investigate the hemodynamic response to somatosensory stimulus in a specific vascular compartment. Compared with the CBF changes in venules, the changes in arterioles and capillaries are more prominent. Such a different CBF response in the arterioles and capillaries could be explained by the received vasodilatory signals in the receptors on capillaries (or capillary dilatation), which then propagate along the vessels to the upstream arteriolar smooth muscle to regulate the blood flow to the neuropil.4244 Therefore, our results suggest that large venules should be avoided when LDF is used to measure the activity-dependent CBF changes.

Effects of Anesthetics

Like most studies on hypothermia, the animals in this study had to be anesthetized throughout the experiments, which could not prevent anesthesia-induced hypothermic hypometabolism. Anesthesia is also unavoidable in clinical hypothermic therapeutics. CBF can be decreased by pentobarbital, according to experimental and clinical studies,45 although such pentobarbital-induced reduction of CBF is homogeneous.46 The extent to which the accumulation of anesthetics can influence the response of CBF under functional stimulation is still unclear. Although no direct evidence exists to prove that the stimulus-induced CBF change is steady during anesthesia, in a study on rats on the effect of anesthetics on functional metabolic coupling in somatosensory stimulation, the regional cerebral metabolic rate of glucose is preserved under pentobarbital anesthesia.47 In addition, the results of Royl et al. 38 did not show significant changes in SEP or CMRO2 under functional stimulation during 3 h of normothermia following the same anesthesia protocols. Nevertheless, a stable anesthesia during the experiment is definitely helpful.

The spatiotemporal neurovascular coupling in brain activity is crucial in understanding the mechanism of brain function under different physiological and pathological conditions. We demonstrated LSI as a promising and quantitative functional imaging method with high spatiotemporal resolution and minimal invasiveness. In particular, using a combination of the two-dimensional LSI technique and TCA method, we were able to detect the high resolution of spatiotemporal CBF changes in response to hindpaw stimulation under hypothermia without any assumed knowledge about the neural activation pattern. The main findings of this study are as follows. 1. Moderate hypothermia delays and prolongs functional activation in the aspect of the relative CBF response, but the activity-induced neuronal activity is preserved. 2. Moderate hypothermia attenuates the maximal activation area and the peak relative CBF change. 3. Capillaries and arterioles are the major contributors in the vascular responses to functional activation.

We thank Ran Luo, Shengfa Zhang, and Jiangwa Xing for their help in the animal experiments. This work was supported by NSFC (Grant No. NSFC 81071192 ).

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Dainty  J. C.,  Laser Speckle and Related Phenomena. ,  Springer-Verlag ,  Berlin and New York  ((1975)).
Dunn  A., , Bolay  H., , Moskowitz  M., , and Boas  D., “ Dynamic imaging of cerebral blood flow using laser speckle. ,” J. Cereb. Blood Flow Metab.. 21, (3 ), 195–201  ((2001)).
Boas  D. A., and Dunn  A. K., “ Laser speckle contrast imaging in biomedical optics. ,” J. Biomed. Opt.. 15, (1 ), 011109  ((2010)).
Bandyopadhyay  R., , Gittings  A., , Suh  S., , Dixon  P., , and Durian  D., “ Speckle-visibility spectroscopy: a tool to study time-varying dynamics. ,” Rev. Sci. Instrum.. 76, , 093110  ((2005)).
Ramirez-San-Juan  J. C., , Ramos-GarcYa  R., , Guizar-Iturbide  I., , MartYnez-Niconoff  G., , and Choi  B., “ Impact of velocity distribution assumption on simplified laser speckle imaging equation. ,” Opt. Express. 16, (5 ), 3197–3203  ((2008)).
Duncan  D. D., and Kirkpatrick  S. J., “ Can laser speckle flowmetry be made a quantitative tool. ?,” J. Opt. Soc. Am. A. 25, (8 ), 2088–2094  ((2008)).
Liu  Y., , Gao  J. H., , Liu  H. L., , and Fox  P. T., “ The temporal response of the brain after eating revealed by functional MRI. ,” Nature. 405, (6790 ), 1058–1062  ((2000)).
Yee  S. H., and Gao  J. H., “ Improved detection of time windows of brain responses in fMRI using modified temporal clustering analysis. ,” Magn. Reson. Imaging. 20, (1 ), 17–26  ((2002)).
Steinmeier  R., , Bondar  I., , Bauhuf  C., , and Fahlbusch  R., “ Laser Doppler flowmetry mapping of cerebrocortical microflow: characteristics and limitations. ,” Neuroimage. 15, (1 ), 107–119  ((2002)).
Lau  W., , Tong  S., , and Thakor  N. V., “ Spatiotemporal characteristics of low-frequency functional activation measured by laser speckle imaging. ,” IEEE Trans. Neural Syst. Rehabil. Eng.. 13, (2 ), 179–185  ((2005)).
Hillman  E., , Devor  A., , Bouchard  M. B., , Dunn  A. K., , Krauss  G. W., , Skoch  J., , Bacskai  B. J., , Dale  A. M., , and Boas  D. A., “ Depth-resolved optical imaging and microscopy of vascular compartment dynamics during somatosensory stimulation. ,” Neuroimage. 35, (1 ), 89–104  ((2007)).
Durduran  T., , Burnett  M. G., , Yu  G., , Zhou  C., , Furuya  D., , Yodh  A. G., , Detre  J. A., , and Greenberg  J. H., “ Spatiotemporal quantification of cerebral blood flow during functional activation in rat somatosensory cortex using laser-speckle flowmetry. ,” J. Cereb. Blood Flow Metab.. 24, (5 ), 518–525  ((2004)).
Sheth  S. A., , Nemoto  M., , Guiou  M., , Walker  M., , Pouratian  N., , and Toga  A. W., “ Linear and nonlinear relationships between neuronal activity, oxygen metabolism, and hemodynamic responses. ,” Neuron. 42, (2 ), 347–355  ((2004)).
Rajapakse  J. C., , Kruggel  F., , Maisog  J. M., , and Von Cramon  D. Y., “ Modeling hemodynamic response for analysis of functional MRI time-series. ,” Hum. Brain Mapp. 6, (4 ), 283–300  ((1998)).
Svensen  M., , Kruggel  F., , and Von Cramon  D. Y., “ Probabilistic modeling of single-trial fMRI data. ,” IEEE Trans. Med. Imaging. 19, (1 ), 25–35  ((2002)).
Dripps  R. D., and Virtue  R. W., “ The physiology of induced hypothermia. Proceedings of a symposium. ,” Anesthesiology. 19, (1 ), 118–119  ((1958)).
Kiyatkin  E. A., “ Brain hyperthermia as physiological and pathological phenomena. ,” Brain Res. Rev.. 50, (1 ), 27–56  ((2005)).
Royl  G., , Füchtemeier  M., , Leithner  C., , Megow  D., , Offenhauser  N., , Steinbrink  J., , Kohl-Bareis  M., , Dirnagl  U., , and Lindauer  U., “ Hypothermia effects on neurovascular coupling and cerebral metabolic rate of oxygen. ,” Neuroimage. 40, (4 ), 1523–1532  ((2008)).
Deboer  T., and Tobler  I., “ Temperature dependence of EEG frequencies during natural hypothermia. ,” Brain Res.. 670, (1 ), 153–156  ((1995)).
Zhu  X. H., , Zhang  Y., , Zhang  N., , Ugurbil  K., , and Chen  W., “ Noninvasive and three-dimensional imaging of CMRO2 in rats at 9.4 T: reproducibility test and normothermia/hypothermia comparison study. ,” J. Cereb. Blood Flow Metab.. 27, (6 ), 1225–1234  ((2006)).
Krafft  P., , Frietsch  T., , Lenz  C., , Piepgras  A., , Kuschinsky  W., , Waschke  K. F., , and Iadecola  C., “ Mild and moderate hypothermia ({alpha}-stat) do not impair the coupling between local cerebral blood flow and metabolism in rats editorial comment. ,” Stroke. 31, (6 ), 1393–1400  ((2000)).
Duelli  R., and Kuschinsky  W., “ Changes in brain capillary diameter during hypocapnia and hypercapnia. ,” J. Cereb. Blood Flow Metab.. 13, (6 ), 1025–1028  ((1993)).
Iadecola  C., , Yang  G., , Ebner  T. J., , and Chen  G., “ Local and propagated vascular responses evoked by focal synaptic activity in cerebellar cortex. ,” J. Neurophysiol.. 78, (2 ), 651–659  ((1997)).
Villringer  A., , Them  A., , Lindauer  U., , Einhaupl  K., , and Dirnagl  U., “ Capillary perfusion of the rat brain cortex. An in vivo confocal microscopy study. ,” Circ. Res.. 75, (1 ), 55–62  ((1994)).
Hendrich  K. S., , Kochanek  P. M., , Melick  J. A., , Schiding  J. K., , Statler  K. D., , Williams  D. S., , Marion  D. W., , and Ho  C., “ Cerebral perfusion during anesthesia with fentanyl, isoflurane, or pentobarbital in normal rats studied by arterial spin-labeled MRI. ,” Magn. Reson. Med.. 46, (1 ), 202–206  ((2001)).
Gjedde  A., and Rasmussen  M., “ Blood-brain glucose transport in the conscious rat: comparison of the intravenous and intracarotid injection methods. ,” J. Neurochem.. 35, (6 ), 1375–1381  ((1980)).
Ueki  M., , Mies  G., , and Hossmann  K. A., “ Effect of alpha-chloralose, halothane, pentobarbital and nitrous oxide anesthesia on metabolic coupling in somatosensory cortex of rat. ,” Acta Anaesthesiol.Scand.. 36, (4 ), 318–322  ((1992)).
© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Minheng Li ; Peng Miao ; Yisheng Zhu and Shanbao Tong
"Functional laser speckle imaging of cerebral blood flow under hypothermia", J. Biomed. Opt. 16(8), 086011 (August 01, 2011). ; http://dx.doi.org/10.1117/1.3610995


Figures

Graphic Jump LocationF1 :

Relative changes in CBF response to 4 s hindpaw stimulation in a typical animal under normothermia (37°C). The CBF changes higher than 3% are overlaid over the vasculature derived by speckle contrast images. Each image represents a 500 ms interval. A, anterior; P, posterior; L, lateral.

Graphic Jump LocationF2 :

Relative changes in CBF response to 4 s hindpaw stimulation in a typical animal under hypothermia (32°C). The CBF changes higher than 3% are overlaid over the vasculature derived by speckle contrast images. Each image represents a 500 ms interval. A, anterior; P, posterior; L, lateral.

Graphic Jump LocationF3 :

Spatiotemporal CBF changes response to the hindpaw stimulation under normothermia (37°C) and hypothermia (32°C). (a) Peak relative CBF change response to the hindpaw stimulation occurring 3 s after the stimulus onset under normothermia. (b) Peak relative CBF change response to the hindpaw stimulation occurring 5 s after the stimulus onset under hypothermia. The white boxes in (a) and (b) denote the ROI in measuring the spatial properties of the brain activation. (c) Temporal cluster of CBF response to the hindpaw stimulation. The TCA results are fitted with the Gaussian function (dotted line) used to detect the activation time window. For normothermia (37°C) (the red part), a time window (1.5 to 4.3 s) centered at tmax (2.9 s) is determined by the Gaussian fitting (r = 0.9511). For hypothermia (32°C) (the blue part), a time window (2.5 to 7.9 s) centered at tmax (5.2 s) is estimated by the Gaussian fitting (r = 0.9269). A, anterior; P, posterior; L, lateral.

Graphic Jump LocationF4 :

Scatter plots showing the correlation of the (a) response peak (tmax) and (b) response duration (Rd) between normothermic and hypothermic stages. The best-fit line and correlation coefficient (R2) values represent the results of the linear regression analysis.

Graphic Jump LocationF5 :

Scatter plots showing the correlation of the peak relative CBF change (

ΔCBFtmax) between normothermic and hypothermic stages. The best-fit line and correlation coefficient (R2) values represent the results of the linear regression analysis.

Graphic Jump LocationF6 :

Blood flow change in rat's primary somatosensory cortex under hindpaw stimulation. Three ROI are selected for analyzing the CBF changes in different vessels; i.e., (a) whole activated area (ROI A) with two subregions for the capillary bed and arteriole (ROI B) and venule (ROI C). The CBF change in each rat is normalized by the maximum value of ROI B. The error bars represent the standard deviation. (b) Relative CBF changes in ROI. A, anterior; P, posterior; L, lateral.

Tables

Table Grahic Jump Location
HR and BP under normothermia and hypothermia. (Data are presented as mean ± SD; *: paired t-test with hypothermia, P < 0.05.)
Table Grahic Jump Location
Spatial and temporal responses to 4 s hindpaw stimulation under normothermia and hypothermia (n = 8) in the aspect of relative CBF change. The time scale starts from the onset of a stimulus. (Data are presented as mean ± SD; *: paired t-test with hypothermia, P < 0.05.)

References

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Kim  S. H., , Stezoski  S. W., , Safar  P., , Capone  A., , and Tisherman  S., “ Hypothermia and minimal fluid resuscitation increase survival after uncontrolled hemorrhagic shock in rats. ,” J. Trauma: Inj., Infect., Crit. Care. 42, (2 ), 213–222  ((1997)).
Goodman  J. W., “ Some fundamental properties of speckle. ,” J. Opt. Soc. Am.. 66, (11 ), 1145–1150  ((1976)).
Dainty  J. C.,  Laser Speckle and Related Phenomena. ,  Springer-Verlag ,  Berlin and New York  ((1975)).
Dunn  A., , Bolay  H., , Moskowitz  M., , and Boas  D., “ Dynamic imaging of cerebral blood flow using laser speckle. ,” J. Cereb. Blood Flow Metab.. 21, (3 ), 195–201  ((2001)).
Boas  D. A., and Dunn  A. K., “ Laser speckle contrast imaging in biomedical optics. ,” J. Biomed. Opt.. 15, (1 ), 011109  ((2010)).
Bandyopadhyay  R., , Gittings  A., , Suh  S., , Dixon  P., , and Durian  D., “ Speckle-visibility spectroscopy: a tool to study time-varying dynamics. ,” Rev. Sci. Instrum.. 76, , 093110  ((2005)).
Ramirez-San-Juan  J. C., , Ramos-GarcYa  R., , Guizar-Iturbide  I., , MartYnez-Niconoff  G., , and Choi  B., “ Impact of velocity distribution assumption on simplified laser speckle imaging equation. ,” Opt. Express. 16, (5 ), 3197–3203  ((2008)).
Duncan  D. D., and Kirkpatrick  S. J., “ Can laser speckle flowmetry be made a quantitative tool. ?,” J. Opt. Soc. Am. A. 25, (8 ), 2088–2094  ((2008)).
Liu  Y., , Gao  J. H., , Liu  H. L., , and Fox  P. T., “ The temporal response of the brain after eating revealed by functional MRI. ,” Nature. 405, (6790 ), 1058–1062  ((2000)).
Yee  S. H., and Gao  J. H., “ Improved detection of time windows of brain responses in fMRI using modified temporal clustering analysis. ,” Magn. Reson. Imaging. 20, (1 ), 17–26  ((2002)).
Steinmeier  R., , Bondar  I., , Bauhuf  C., , and Fahlbusch  R., “ Laser Doppler flowmetry mapping of cerebrocortical microflow: characteristics and limitations. ,” Neuroimage. 15, (1 ), 107–119  ((2002)).
Lau  W., , Tong  S., , and Thakor  N. V., “ Spatiotemporal characteristics of low-frequency functional activation measured by laser speckle imaging. ,” IEEE Trans. Neural Syst. Rehabil. Eng.. 13, (2 ), 179–185  ((2005)).
Hillman  E., , Devor  A., , Bouchard  M. B., , Dunn  A. K., , Krauss  G. W., , Skoch  J., , Bacskai  B. J., , Dale  A. M., , and Boas  D. A., “ Depth-resolved optical imaging and microscopy of vascular compartment dynamics during somatosensory stimulation. ,” Neuroimage. 35, (1 ), 89–104  ((2007)).
Durduran  T., , Burnett  M. G., , Yu  G., , Zhou  C., , Furuya  D., , Yodh  A. G., , Detre  J. A., , and Greenberg  J. H., “ Spatiotemporal quantification of cerebral blood flow during functional activation in rat somatosensory cortex using laser-speckle flowmetry. ,” J. Cereb. Blood Flow Metab.. 24, (5 ), 518–525  ((2004)).
Sheth  S. A., , Nemoto  M., , Guiou  M., , Walker  M., , Pouratian  N., , and Toga  A. W., “ Linear and nonlinear relationships between neuronal activity, oxygen metabolism, and hemodynamic responses. ,” Neuron. 42, (2 ), 347–355  ((2004)).
Rajapakse  J. C., , Kruggel  F., , Maisog  J. M., , and Von Cramon  D. Y., “ Modeling hemodynamic response for analysis of functional MRI time-series. ,” Hum. Brain Mapp. 6, (4 ), 283–300  ((1998)).
Svensen  M., , Kruggel  F., , and Von Cramon  D. Y., “ Probabilistic modeling of single-trial fMRI data. ,” IEEE Trans. Med. Imaging. 19, (1 ), 25–35  ((2002)).
Dripps  R. D., and Virtue  R. W., “ The physiology of induced hypothermia. Proceedings of a symposium. ,” Anesthesiology. 19, (1 ), 118–119  ((1958)).
Kiyatkin  E. A., “ Brain hyperthermia as physiological and pathological phenomena. ,” Brain Res. Rev.. 50, (1 ), 27–56  ((2005)).
Royl  G., , Füchtemeier  M., , Leithner  C., , Megow  D., , Offenhauser  N., , Steinbrink  J., , Kohl-Bareis  M., , Dirnagl  U., , and Lindauer  U., “ Hypothermia effects on neurovascular coupling and cerebral metabolic rate of oxygen. ,” Neuroimage. 40, (4 ), 1523–1532  ((2008)).
Deboer  T., and Tobler  I., “ Temperature dependence of EEG frequencies during natural hypothermia. ,” Brain Res.. 670, (1 ), 153–156  ((1995)).
Zhu  X. H., , Zhang  Y., , Zhang  N., , Ugurbil  K., , and Chen  W., “ Noninvasive and three-dimensional imaging of CMRO2 in rats at 9.4 T: reproducibility test and normothermia/hypothermia comparison study. ,” J. Cereb. Blood Flow Metab.. 27, (6 ), 1225–1234  ((2006)).
Krafft  P., , Frietsch  T., , Lenz  C., , Piepgras  A., , Kuschinsky  W., , Waschke  K. F., , and Iadecola  C., “ Mild and moderate hypothermia ({alpha}-stat) do not impair the coupling between local cerebral blood flow and metabolism in rats editorial comment. ,” Stroke. 31, (6 ), 1393–1400  ((2000)).
Duelli  R., and Kuschinsky  W., “ Changes in brain capillary diameter during hypocapnia and hypercapnia. ,” J. Cereb. Blood Flow Metab.. 13, (6 ), 1025–1028  ((1993)).
Iadecola  C., , Yang  G., , Ebner  T. J., , and Chen  G., “ Local and propagated vascular responses evoked by focal synaptic activity in cerebellar cortex. ,” J. Neurophysiol.. 78, (2 ), 651–659  ((1997)).
Villringer  A., , Them  A., , Lindauer  U., , Einhaupl  K., , and Dirnagl  U., “ Capillary perfusion of the rat brain cortex. An in vivo confocal microscopy study. ,” Circ. Res.. 75, (1 ), 55–62  ((1994)).
Hendrich  K. S., , Kochanek  P. M., , Melick  J. A., , Schiding  J. K., , Statler  K. D., , Williams  D. S., , Marion  D. W., , and Ho  C., “ Cerebral perfusion during anesthesia with fentanyl, isoflurane, or pentobarbital in normal rats studied by arterial spin-labeled MRI. ,” Magn. Reson. Med.. 46, (1 ), 202–206  ((2001)).
Gjedde  A., and Rasmussen  M., “ Blood-brain glucose transport in the conscious rat: comparison of the intravenous and intracarotid injection methods. ,” J. Neurochem.. 35, (6 ), 1375–1381  ((1980)).
Ueki  M., , Mies  G., , and Hossmann  K. A., “ Effect of alpha-chloralose, halothane, pentobarbital and nitrous oxide anesthesia on metabolic coupling in somatosensory cortex of rat. ,” Acta Anaesthesiol.Scand.. 36, (4 ), 318–322  ((1992)).

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