Research Papers: Imaging

Protoporphyrin IX fluorescence contrast in invasive glioblastomas is linearly correlated with Gd enhanced magnetic resonance image contrast but has higher diagnostic accuracy

[+] Author Affiliations
Kimberley S. Samkoe, P. Jack Hoopes, Brian W. Pogue

Dartmouth College, Thayer School of Engineering, Hanover New Hampshire 03755

Dartmouth Medical School, Department of Surgery, Lebanon, New Hampshire 03756

Summer L. Gibbs-Strauss, Harold H. Yang

Dartmouth College, Thayer School of Engineering, Hanover New Hampshire 03755

S. Khan Hekmatyar, Risto A. Kauppinen

Dartmouth Medical School, Department of Diagnostic Radiology, Hanover, New Hampshire 03755

Julia A. O’Hara

Dartmouth College, Thayer School of Engineering, Hanover New Hampshire 03755

Dartmouth Medical School, Department of Diagnostic Radiology, Hanover, New Hampshire 03755

J. Biomed. Opt. 16(9), 096008 (September 01, 2011). doi:10.1117/1.3622754
History: Received May 04, 2011; Revised July 15, 2011; Accepted July 18, 2011; Published September 01, 2011; Online September 01, 2011
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The sensitivity and specificity of in vivo magnetic resonance (MR) imaging is compared with production of protoporphyrin IX (PpIX), determined ex vivo, in a diffusely infiltrating glioma. A human glioma transfected with green fluorescent protein, displaying diffuse, infiltrative growth, was implanted intracranially in athymic nude mice. Image contrast from corresponding regions of interest (ROIs) in in vivo MR and ex vivo fluorescence images was quantified. It was found that all tumor groups had statistically significant PpIX fluorescence contrast and that PpIX contrast demonstrated the best predictive power for tumor presence. Contrast from gadolinium enhanced T1-weighted (T1W+Gd) and absolute T2 images positively predicted the presence of a tumor, confirmed by the GFP positive (GFP+) and hematoxylin and eosin positive (H&E+) ROIs. However, only the absolute T2 images had predictive power from controls in ROIs that were GFP+ but H&E negative. Additionally, PpIX fluorescence and T1W+Gd image contrast were linearly correlated in both the GFP+ (r = 0.79, p<1×10−8) and H&E+ (r = 0.74, p<0.003) ROIs. The trace diffusion images did not have predictive power or significance from controls. This study indicates that gadolinium contrast enhanced MR images can predict the presence of diffuse tumors, but PpIX fluorescence is a better predictor regardless of tumor vascularity.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Kimberley S. Samkoe ; Summer L. Gibbs-Strauss ; Harold H. Yang ; S. Khan Hekmatyar ; P. Jack Hoopes, et al.
"Protoporphyrin IX fluorescence contrast in invasive glioblastomas is linearly correlated with Gd enhanced magnetic resonance image contrast but has higher diagnostic accuracy", J. Biomed. Opt. 16(9), 096008 (September 01, 2011). ; http://dx.doi.org/10.1117/1.3622754


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