Research Papers: Imaging

Lipidots: competitive organic alternative to quantum dots for in vivo fluorescence imaging

[+] Author Affiliations
Julien Gravier, Fabrice P. Navarro, Thomas Delmas, Frédérique Mittler, Anne-Claude Couffin, Françoise Vinet, Isabelle Texier

CEA Grenoble, LETI-DTBS, MINATEC Campus, Grenoble, 38054 France

J. Biomed. Opt. 16(9), 096013 (September 14, 2011). doi:10.1117/1.3625405
History: Received April 13, 2011; Revised July 08, 2011; Accepted July 22, 2011; Published September 14, 2011; Online September 14, 2011
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The use of fluorescent nanostructures can bring several benefits on the signal to background ratio for in vitro microscopy, in vivo small animal imaging, and image-guided surgery. Fluorescent quantum dots (QDs) display outstanding optical properties, with high brightness and low photobleaching rate. However, because of their toxic element core composition and their potential long term retention in reticulo-endothelial organs such as liver, their in vivo human applications seem compromised. The development of new dye-loaded (DiO, DiI, DiD, DiR, and Indocyanine Green (ICG)) lipid nanoparticles for fluorescence imaging (lipidots) is described here. Lipidot optical properties quantitatively compete with those of commercial QDs (QTracker®705). Multichannel in vivo imaging of lymph nodes in mice is demonstrated for doses as low as 2 pmols of particles. Along with their optical properties, fluorescent lipidots display very low cytotoxicity (IC50 > 75 nM), which make them suitable tools for in vitro, and especially in vivo, fluorescence imaging applications.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Julien Gravier ; Fabrice P. Navarro ; Thomas Delmas ; Frédérique Mittler ; Anne-Claude Couffin, et al.
"Lipidots: competitive organic alternative to quantum dots for in vivo fluorescence imaging", J. Biomed. Opt. 16(9), 096013 (September 14, 2011). ; http://dx.doi.org/10.1117/1.3625405


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