Research Papers: Imaging

Spectrally resolved multidepth fluorescence imaging

[+] Author Affiliations
Yuan Luo

Massachusetts Institute of Technology, Department of Mechanical Engineering, Cambridge, Massachusetts 02139

National Taiwan University, College of Medicine, Center for Optoelectronic Biomedicine, 10051, Taiwan

Ioannis K. Zervantonakis, Se Baek Oh

Massachusetts Institute of Technology, Department of Mechanical Engineering, Cambridge, Massachusetts 02139

Roger D. Kamm

Massachusetts Institute of Technology, Department of Mechanical Engineering, Cambridge, Massachusetts 02139

Massachusetts Institute of Technology, Department of Biological Engineering, Cambridge, Massachusetts 02139

Singapore-MIT Alliance for Research and Technology (SMART) Centre, 117543 Singapore

George Barbastathis

Massachusetts Institute of Technology, Department of Mechanical Engineering, Cambridge, Massachusetts 02139

Singapore-MIT Alliance for Research and Technology (SMART) Centre, 117543 Singapore

J. Biomed. Opt. 16(9), 096015 (September 09, 2011). doi:10.1117/1.3626211
History: Received March 20, 2011; Revised July 15, 2011; Accepted July 27, 2011; Published September 09, 2011; Online September 09, 2011
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We present a multicolor fluorescence imaging modality to visualize in real-time tissue structures emitting multispectral fluorescent light from different focal depths. Each designated spectrum of fluorescent emission from a specific depth within a volumetric tissue is probed by a depth-spectrum selective holographic grating. The grating for each fluorescent color are multiplexed within a volume hologram, which enables simultaneously obtaining multicolored fluorescent information at different depths within a biological tissue sample. We demonstrate the imaging modality's ability to obtain laser-induced multicolored fluorescence images of a biological sample from different depths without scanning. We also experimentally demonstrate that the imaging modality can be simultaneously operated at both fluorescent and bright field modes to provide complementary information of volumetric tissue structures at different depths in real-time.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Yuan Luo ; Ioannis K. Zervantonakis ; Se Baek Oh ; Roger D. Kamm and George Barbastathis
"Spectrally resolved multidepth fluorescence imaging", J. Biomed. Opt. 16(9), 096015 (September 09, 2011). ; http://dx.doi.org/10.1117/1.3626211


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