Research Papers: Imaging

Quantitative photoacoustic imaging: correcting for heterogeneous light fluence distributions using diffuse optical tomography

[+] Author Affiliations
Adam Q. Bauer, Ralph E. Nothdurft

Washington University School of Medicine, Department of Radiology, St. Louis, Missouri 63110

Todd N. Erpelding

Philips Research North America, 345 Scarborough Rd., Briarcliff Manor, New York 10510

Lihong V. Wang

Washington University in Saint Louis, Department of Biomedical Engineering, St. Louis, Missouri 63130

Joseph P. Culver

Washington University School of Medicine, Department of Radiology, St. Louis, Missouri 63110

Washington University in Saint Louis, Department of Biomedical Engineering, St. Louis, Missouri 63130

J. Biomed. Opt. 16(9), 096016 (September 01, 2011). doi:10.1117/1.3626212
History: Received March 18, 2011; Revised July 27, 2011; Accepted July 29, 2011; Published September 01, 2011; Online September 01, 2011
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The specificity of molecular and functional photoacoustic (PA) images depends on the accuracy of the photoacoustic absorption spectroscopy. The PA signal is proportional to the product of the optical absorption coefficient and local light fluence; quantitative PA measurements of the optical absorption coefficient therefore require an accurate estimation of optical fluence. Light-modeling aided by diffuse optical tomography (DOT) can be used to map the required fluence and to reduce errors in traditional PA spectroscopic analysis. As a proof-of-concept, we designed a tissue-mimicking phantom to demonstrate how fluence-related artifacts in PA images can lead to misrepresentations of tissue properties. To correct for these inaccuracies, the internal fluence in the tissue phantom was estimated by using DOT to reconstruct spatial distributions of the absorption and reduced scattering coefficients of multiple targets within the phantom. The derived fluence map, which only consisted of low spatial frequency components, was used to correct PA images of the phantom. Once calibrated to a known absorber, this method reduced errors in estimated absorption coefficients from 33% to 6%. These results experimentally demonstrate that combining DOT with PA imaging can significantly reduce fluence-related errors in PA images, while producing quantitatively accurate, high-resolution images of the optical absorption coefficient.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Adam Q. Bauer ; Ralph E. Nothdurft ; Todd N. Erpelding ; Lihong V. Wang and Joseph P. Culver
"Quantitative photoacoustic imaging: correcting for heterogeneous light fluence distributions using diffuse optical tomography", J. Biomed. Opt. 16(9), 096016 (September 01, 2011). ; http://dx.doi.org/10.1117/1.3626212


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