Research Papers: Imaging

Initial results of imaging melanoma metastasis in resected human lymph nodes using photoacoustic computed tomography

[+] Author Affiliations
Jithin Jose, Diederik J. Grootendorst, Thomas W. Vijn, Wiendelt Steenbergen, Srirang Manohar

University of Twente, MIRA–Institute for Biomedical Technology and Technical Medicine, Biomedical Photonic Imaging Group, P.O. Box 217, AE Enschede, 7500 The Netherlands

Michel W. Wouters

Netherlands Cancer Institute—Antoni van Leeuwenhoek Hospital (NKI-AVL), Department of Surgical Oncology, P.O. Box 90203, BE Amsterdam, 1006 The Netherlands

Hester van Boven

Netherlands Cancer Institute—Antoni van Leeuwenhoek Hospital (NKI-AVL), Department of Pathology, P.O. Box 90203, BE Amsterdam, 1006 The Netherlands

Ton G. van Leeuwen

University of Twente, MIRA–Institute for Biomedical Technology and Technical Medicine, Biomedical Photonic Imaging Group, P.O. Box 217, AE Enschede, 7500 The Netherlands

University of Amsterdam, Academic Medical Center, Biomedical Engineering and Physics, P.O. Box 22700, DE, Amsterdam, 1100 The Netherlands

Theo J. M. Ruers

Netherlands Cancer Institute—Antoni van Leeuwenhoek Hospital (NKI-AVL), Department of Surgical Oncology, P.O. Box 90203, BE Amsterdam, 1006 The Netherlands

University of Twente, MIRA–Institute for Biomedical Technology and Technical Medicine, P.O. Box 217, AE Enschede, 7500 The Netherlands

J. Biomed. Opt. 16(9), 096021 (September 23, 2011). doi:10.1117/1.3631705
History: Received May 31, 2011; Revised August 07, 2011; Accepted August 10, 2011; Published September 23, 2011; October 27, 2011; Online September 23, 2011
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The pathological status of the sentinel lymph node is important for accurate melanoma staging, ascertaining prognosis and planning treatment. The standard procedure involves biopsy of the node and histopathological assessment of its status. Drawbacks of this examination include a finite sampling of the node with the likelihood of missing metastases, and a significant time-lag before histopathological results are available to the surgeon. We studied the applicability of photoacoustic computed tomographic imaging as an intraoperative modality for examining the status of resected human sentinel lymph nodes. We first applied the technique to image ex vivo pig lymph nodes carrying metastases-simulating melanoma cells using multiple wavelengths. The experience gained was applied to image a suspect human lymph node. We validated the photoacoustic imaging results by comparing a reconstructed slice with a histopathological section through the node. Our results suggest that photoacoustics has the potential to develop into an intraoperative imaging method to detect melanoma metastases in sentinel lymph nodes.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Jithin Jose ; Diederik J. Grootendorst ; Thomas W. Vijn ; Michel W. Wouters ; Hester van Boven, et al.
"Initial results of imaging melanoma metastasis in resected human lymph nodes using photoacoustic computed tomography", J. Biomed. Opt. 16(9), 096021 (September 23, 2011). ; http://dx.doi.org/10.1117/1.3631705


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