Research Papers: General

Trapping and two-photon fluorescence excitation of microscopic objects using ultrafast single-fiber optical tweezers

[+] Author Affiliations
Yogeshwar N. Mishra

University of Texas at Arlington, Department of Physics, Arlington, Texas 76019

Centre of Excellence in Lasers and Optoelectronic Sciences, CUSAT, Cochin 682022, India

Ninad Ingle, Samarendra K. Mohanty

University of Texas at Arlington, Department of Physics, Arlington, Texas 76019

J. Biomed. Opt. 16(10), 105003 (October 03, 2011). doi:10.1117/1.3643340
History: Received June 02, 2011; Revised August 25, 2011; Accepted August 26, 2011; Published October 03, 2011; Online October 03, 2011
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Analysis of trapped microscopic objects using fluorescence and Raman spectroscopy is gaining considerable interest. We report on the development of single fiber ultrafast optical tweezers and its use in simultaneous two-photon fluorescence (TPF) excitation of trapped fluorescent microscopic objects. Using this method, trapping depth of a few centimeters was achieved inside a colloidal sample with TPF from the trapped particle being visible to the naked eye. Owing to the propagation distance of the Bessel-like beam emerging from the axicon-fiber tip, a relatively longer streak of fluorescence was observed along the microsphere length. The cone angle of the axicon was engineered so as to provide better trapping stability and high axial confinement of TPF. Trapping of the floating objects led to stable fluorescence emission intensity over a long period of time, suitable for spectroscopic measurements. Furthermore, the stability of the fiber optic trapping was confirmed by holding and maneuvering the fiber by hand so as to move the trapped fluorescent particle in three dimensions. Apart from miniaturization capability into lab-on-a-chip microfluidic devices, the proposed noninvasive microaxicon tipped optical fiber can be used in multifunctional mode for in-depth trapping, rotation, sorting, and ablation, as well as for two-photon fluorescence excitation of a motile sample.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Yogeshwar N. Mishra ; Ninad Ingle and Samarendra K. Mohanty
"Trapping and two-photon fluorescence excitation of microscopic objects using ultrafast single-fiber optical tweezers", J. Biomed. Opt. 16(10), 105003 (October 03, 2011). ; http://dx.doi.org/10.1117/1.3643340


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