Research Papers: General

Detachment and reorientation of cells using near-infrared laser microbeam

[+] Author Affiliations
Ling Gu

University of Texas at Arlington, Department of Physics, Arlington, Texas 76019

Ninad Ingle

University of Texas at Arlington, Department of Material Science and Engineering, Arlington, Texas 76019

Samarendra K. Mohanty

University of Texas at Arlington, Department of Physics, Arlington, Texas 76019

University of Texas at Arlington, Department of Material Science and Engineering, Arlington, Texas 76019

J. Biomed. Opt. 16(11), 115002 (October 31, 2011). doi:10.1117/1.3646207
History: Received May 17, 2011; Revised August 25, 2011; Accepted September 12, 2011; Published October 31, 2011; Online October 31, 2011
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Reorientation of adhering cell(s) with respect to other cell(s) has not been yet possible, thus limiting study of controlled interaction between cells. Here, we report cell detachment upon irradiation with a focused near-infrared laser beam, and reorientation of adherent cells. The detached cell was transported along the axial direction by scattering force and trapped at a higher plane inside the media using the same laser beam by a gravito-optical trap. The trapped cell could then be repositioned by movement of the sample stage and reoriented by rotation of the astigmatic trapping beam. The height at which the cell was stably held was found to depend on the laser beam power. Viability of the detached and manipulated cell was found not to be compromised as confirmed by propidium iodide fluorescence exclusion assay. The reoriented cell was allowed to reattach to the substrate at a controlled distance and orientation with respect to other cells. Further, the cell was found to retain its shape even after multiple detachments and manipulation using the laser beam. This technique opens up new avenues for noncontact modification of cellular orientations that will enable study of intercellular interactions and design of engineered tissue.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Ling Gu ; Ninad Ingle and Samarendra K. Mohanty
"Detachment and reorientation of cells using near-infrared laser microbeam", J. Biomed. Opt. 16(11), 115002 (October 31, 2011). ; http://dx.doi.org/10.1117/1.3646207


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