Research Papers: Imaging

Correction of stain variations in nuclear refractive index of clinical histology specimens

[+] Author Affiliations
Shikhar Uttam, Rajan K. Bista, Yang Liu

University of Pittsburgh, Department of Medicine and Department of Bioengineering, Biomedical Optical Imaging Laboratory (BOIL), Pittsburgh, Pennsylvania 15232

Douglas J. Hartman

University of Pittsburgh School of Medicine, Department of Pathology, Pittsburgh, Pennsylvania 15213

Randall E. Brand

University of Pittsburgh, Department of Medicine, Division of Gastroenterology, Hepatology, and Nutrition, Pittsburgh, Pennsylvania 15232

J. Biomed. Opt. 16(11), 116013 (October 27, 2011). doi:10.1117/1.3650306
History: Received July 05, 2011; Revised August 19, 2011; Accepted September 21, 2011; Published October 27, 2011; Online October 27, 2011
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For any technique to be adopted into a clinical setting, it is imperative that it seamlessly integrates with well-established clinical diagnostic workflow. We recently developed an optical microscopy technique—spatial-domain low-coherence quantitative phase microscopy (SL-QPM) that can extract the refractive index of the cell nucleus from the standard histology specimens on glass slides prepared via standard clinical protocols. This technique has shown great potential in detecting cancer with a better sensitivity than conventional pathology. A major hurdle in the clinical translation of this technique is the intrinsic variation among staining agents used in histology specimens, which limits the accuracy of refractive index measurements of clinical samples. In this paper, we present a simple and easily generalizable method to remove the effect of variations in staining levels on nuclear refractive index obtained with SL-QPM. We illustrate the efficacy of our correction method by applying it to variously stained histology samples from animal model and clinical specimens.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Shikhar Uttam ; Rajan K. Bista ; Douglas J. Hartman ; Randall E. Brand and Yang Liu
"Correction of stain variations in nuclear refractive index of clinical histology specimens", J. Biomed. Opt. 16(11), 116013 (October 27, 2011). ; http://dx.doi.org/10.1117/1.3650306


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