Research Papers: Imaging

Real-time optical gating for three-dimensional beating heart imaging

[+] Author Affiliations
Jonathan M. Taylor, Christopher D. Saunter

Durham University, Centre for Advanced Instrumentation, Department of Physics, South Road, Durham, DH1 3LE United Kingdom

Durham University, Biophysical Sciences Institute, Durham, DH1 3LE United Kingdom

Gordon D. Love, John M. Girkin

Durham University, Centre for Advanced Instrumentation, Department of Physics, South Road, Durham, DH1 3LE United Kingdom

Durham University, Biophysical Sciences Institute, Durham, DH1 3LE United Kingdom

Durham University, Wolfson Research Institute, Queen's Campus, Durham TS17 6BH United Kingdom

Deborah J. Henderson, Bill Chaudhry

Newcastle University, The Institute of Genetic Medicine, Newcastle NE1 3BZ United Kingdom

J. Biomed. Opt. 16(11), 116021 (November 03, 2011). doi:10.1117/1.3652892
History: Received March 10, 2011; Revised September 22, 2011; Accepted September 27, 2011; Published November 03, 2011; Online November 03, 2011
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We demonstrate real-time microscope image gating to an arbitrary position in the cycle of the beating heart of a zebrafish embryo. We show how this can be used for high-precision prospective gating of fluorescence image slices of the moving heart. We also present initial results demonstrating the application of this technique to 3-D structural imaging of the beating embryonic heart.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Jonathan M. Taylor ; Christopher D. Saunter ; Gordon D. Love ; John M. Girkin ; Deborah J. Henderson, et al.
"Real-time optical gating for three-dimensional beating heart imaging", J. Biomed. Opt. 16(11), 116021 (November 03, 2011). ; http://dx.doi.org/10.1117/1.3652892


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Single camera system for multi-wavelength fluorescent imaging in the heart. Conf Proc IEEE Eng Med Biol Soc 2012;2012():3716-9.
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