Research Papers: Imaging

In vivo spectral imaging of different cell types in the small intestine by two-photon excited autofluorescence

[+] Author Affiliations
Regina Orzekowsky-Schroeder, Björn Martensen, Alfred Vogel, Gereon Hüttmann

University of Lübeck, Institute of Biomedical Optics, Peter-Monnik-Weg 4, Lübeck 23562, Schleswig-Holstein Germany

Antje Klinger, Maike Blessenohl, Andreas Gebert

University of Lübeck, Institute of Anatomy, Ratzeburger Allee 160, 23568 Lübeck, Germany

J. Biomed. Opt. 16(11), 116025 (November 15, 2011). doi:10.1117/1.3655587
History: Received January 02, 2011; Revised October 01, 2011; Accepted October 04, 2011; Published November 15, 2011; Online November 15, 2011
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Spectrally resolved two-photon excited autofluorescence imaging is used to distinguish different cell types and functional areas during dynamic processes in the living gut. Excitation and emission spectra of mucosal tissue and tissue components are correlated to spectra of endogenous chromophores. We show that selective excitation with only two different wavelengths within the tuning range of a Ti:sapphire femtosecond laser system yields excellent discrimination between enterocytes, antigen presenting cells and lysosomes based on the excitation and emission properties of their autofluorescence. The method is employed for time-lapse microscopy over up to 8 h. Changes of the spectral signature with the onset of photodamage are demonstrated, and their origin is discussed.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Regina Orzekowsky-Schroeder ; Antje Klinger ; Björn Martensen ; Maike Blessenohl ; Andreas Gebert, et al.
"In vivo spectral imaging of different cell types in the small intestine by two-photon excited autofluorescence", J. Biomed. Opt. 16(11), 116025 (November 15, 2011). ; http://dx.doi.org/10.1117/1.3655587


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