0
Research Papers: Imaging

Lensless multispectral digital in-line holographic microscope

[+] Author Affiliations
James P. Ryle, John T. Sheridan

University College Dublin, Communications and Optoelectronic Research Centre, Belfield, Dublin D4, Ireland

University College Dublin, SFI Strategic Research Cluster in Solar Energy Conversion, Belfield, Dublin D4, Ireland

University College Dublin, School of Electrical, Electronic and Communications Engineering, Belfield, Dublin D4, Ireland

Susan McDonnell

University College Dublin, School of Chemical and Bioprocess Engineering, College of Engineering, Mathematical, and Physical Sciences, Belfield, Dublin D4, Ireland

J. Biomed. Opt. 16(12), 126004 (June 08, 2011September 21, 2011October 20, 2011December 16, 2011December 16, 2011). doi:10.1117/1.3659681
History: Received June 08, 2011; Revised September 21, 2011; Accepted October 20, 2011; Published December 16, 2011; Online December 16, 2011
Text Size: A A A

An compact multispectral digital in-line holographic microscope (DIHM) is developed that emulates Gabor's original holographic principle. Using sources of varying spatial coherence (laser, LED), holographic images of objects, including optical fiber, latex microspheres, and cancer cells, are successfully captured and numerically processed. Quantitative measurement of cell locations and percentage confluence are estimated, and pseudocolor images are also presented. Phase profiles of weakly scattering cells are obtained from the DIHM and are compared to those produced by a commercially available off-axis digital holographic microscope.

© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

James P. Ryle ; Susan McDonnell and John T. Sheridan
"Lensless multispectral digital in-line holographic microscope", J. Biomed. Opt. 16(12), 126004 (June 08, 2011September 21, 2011October 20, 2011December 16, 2011December 16, 2011). ; http://dx.doi.org/10.1117/1.3659681


Access This Article
Sign In to Access Full Content
Please Wait... Processing your request... Please Wait.
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
 
Your Session has timed out. Please sign back in to continue.
Sign In to Access Full Content

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement

Buy this article ($18 for members, $25 for non-members).
Sign In