Research Papers: Imaging

Lensless multispectral digital in-line holographic microscope

[+] Author Affiliations
James P. Ryle, John T. Sheridan

University College Dublin, Communications and Optoelectronic Research Centre, Belfield, Dublin D4, Ireland

University College Dublin, SFI Strategic Research Cluster in Solar Energy Conversion, Belfield, Dublin D4, Ireland

University College Dublin, School of Electrical, Electronic and Communications Engineering, Belfield, Dublin D4, Ireland

Susan McDonnell

University College Dublin, School of Chemical and Bioprocess Engineering, College of Engineering, Mathematical, and Physical Sciences, Belfield, Dublin D4, Ireland

J. Biomed. Opt. 16(12), 126004 (December 16, 2011). doi:10.1117/1.3659681
History: Received June 08, 2011; Revised September 21, 2011; Accepted October 20, 2011; Published December 16, 2011; Online December 16, 2011
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An compact multispectral digital in-line holographic microscope (DIHM) is developed that emulates Gabor's original holographic principle. Using sources of varying spatial coherence (laser, LED), holographic images of objects, including optical fiber, latex microspheres, and cancer cells, are successfully captured and numerically processed. Quantitative measurement of cell locations and percentage confluence are estimated, and pseudocolor images are also presented. Phase profiles of weakly scattering cells are obtained from the DIHM and are compared to those produced by a commercially available off-axis digital holographic microscope.

© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

James P. Ryle ; Susan McDonnell and John T. Sheridan
"Lensless multispectral digital in-line holographic microscope", J. Biomed. Opt. 16(12), 126004 (December 16, 2011). ; http://dx.doi.org/10.1117/1.3659681


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