Research Papers: Sensing

Discriminating adenocarcinoma from normal colonic mucosa through deconvolution of Raman spectra

[+] Author Affiliations
Patricia Cambraia Lopes, Joaquim Agostinho Moreira, Abilio Almeida, Artur Esteves

IFIMUP and IN–Institute of Nanoscience and Nanotechnology, Universidade do Porto, Departamento de Física e Astronomia da Faculdade de Ciências, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal

Ivan Gregora, Martin Ledinsky

Academy of Sciences of the Czech Republic, Institute of Physics, Prague, Czech Republic

Jose Machado Lopes

Portuguese Oncology Institute, Department of Medical Oncology, Porto, Portugal

Rui Henrique

Portuguese Oncology Institute, Department of Pathology, Porto, Portugal

University of Porto, Department of Pathology and Molecular Immunology, Institute of Biomedical Sciences Abel Salazar (ICBAS), Porto, Portugal

Albino Oliveira

São Sebastião Hospital, Pathological Anatomy Laboratory, Espinho, Vila da Feira, Portugal

J. Biomed. Opt. 16(12), 127001 (November 23, 2011). doi:10.1117/1.3658756
History: Received July 17, 2011; Revised October 14, 2011; Accepted October 14, 2011; Published November 23, 2011; Online November 23, 2011
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In this work, we considered the feasibility of Raman spectroscopy for discriminating between adenocarcinomatous and normal mucosal formalin-fixed colonic tissues. Unlike earlier studies in colorectal cancer, a spectral deconvolution model was implemented to derive spectral information. Eleven samples of human colon were used, and 55 spectra were analyzed. Each spectrum was resolved into 25 bands from 975 to 1720 cm−1, where modes of proteins, lipids, and nucleic acids are observed. From a comparative study of band intensities, those presenting higher differences between tissue types were correlated to biochemical assignments. Results from fitting procedure were further used as inputs for linear discriminant analysis, where combinations of band intensities and intensity ratios were tested, yielding accuracies up to 81%. This analysis yields objective discriminating parameters after fitting optimization. The bands with higher diagnosis relevance detected by spectra deconvolution enable to confine the study to some spectral regions instead of broader ranges. A critical view upon limitations of this approach is presented, along with a comparison of our results to earlier ones obtained in fresh colonic tissues. This enabled to assess the effect of formalin fixation in colonic tissues, and determine its relevance in the present analysis.

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© 2011 Society of Photo-Optical Instrumentation Engineers (SPIE)

Citation

Patricia Cambraia Lopes ; Joaquim Agostinho Moreira ; Abilio Almeida ; Artur Esteves ; Ivan Gregora, et al.
"Discriminating adenocarcinoma from normal colonic mucosa through deconvolution of Raman spectra", J. Biomed. Opt. 16(12), 127001 (November 23, 2011). ; http://dx.doi.org/10.1117/1.3658756


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