Research Papers: General

Optical clearing of porcine skin tissue in vitro studied by Raman microspectroscopy

[+] Author Affiliations
Deqiu Huang, Wen Zhang, Huiqing Zhong, Honglian Xiong, Xi Guo, Zhouyi Guo

South China Normal University, Laboratory of Photonic Chinese Medicine, MOE Key Laboratory of Laser Life Science, College of Biophotonics, 510631, Guangzhou, Guangdong, China

J. Biomed. Opt. 17(1), 015004 (Feb 06, 2012). doi:10.1117/1.JBO.17.1.015004
History: Received August 27, 2011; Revised October 26, 2011; Accepted November 8, 2011
Text Size: A A A

Abstract.  In present work, we studied the effect of optical clearing on porcine skin in vitro with glycerol by Raman microspectroscopy, denoted as RM, at various time intervals of 0, 15, 30, 45, 60, and 75 min respectively. The results showed that the addition of glycerol significantly improved the depth of RM measurement, and enhanced the recovery of skin tissue Raman spectra that were not overlapped with the glycerol Raman spectra over time. Moreover, it was found that the Raman signals resembled the native spectrum of the molecules in porcine skin with a negligible frequency shift. Furthermore, we evaluated the extent of optical clearing in porcine skin by utilizing various concentrations of 40%, 60%, and 80% glycerol solution. The results demonstrated that with the increase of concentration of glycerol, the optical clearing of porcine skin was much improved.

Figures in this Article
© 2012 Society of Photo-Optical Instrumentation Engineers

Citation

Deqiu Huang ; Wen Zhang ; Huiqing Zhong ; Honglian Xiong ; Xi Guo, et al.
"Optical clearing of porcine skin tissue in vitro studied by Raman microspectroscopy", J. Biomed. Opt. 17(1), 015004 (Feb 06, 2012). ; http://dx.doi.org/10.1117/1.JBO.17.1.015004


Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).

Some tools below are only available to our subscribers or users with an online account.

Related Content

Customize your page view by dragging & repositioning the boxes below.

Related Book Chapters

Topic Collections

PubMed Articles
Advertisement
  • Don't have an account?
  • Subscribe to the SPIE Digital Library
  • Create a FREE account to sign up for Digital Library content alerts and gain access to institutional subscriptions remotely.
Access This Article
Sign in or Create a personal account to Buy this article ($20 for members, $25 for non-members).
Access This Proceeding
Sign in or Create a personal account to Buy this article ($15 for members, $18 for non-members).
Access This Chapter

Access to SPIE eBooks is limited to subscribing institutions and is not available as part of a personal subscription. Print or electronic versions of individual SPIE books may be purchased via SPIE.org.